ArticlePloS one2016
Controlling Growth and Osteogenic Differentiation of Osteoblasts on Microgrooved Polystyrene Surfaces.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Antibacterial Titanium Implants Biofunctionalized by Plasma Electrolytic Oxidation with Silver, Zinc, and Copper: A Systematic Review.International journal of molecular sciences · 2021Pooled it
- Topographical design principles for osteochondral tissue engineering.Bioactive materials · 2026Review
- Effects of Multiple Near-Infrared LEDs (700, 850, and 980 nm) CW-PBM on Mitochondrial Respiration and Gene Expression in MG63 Osteoblasts.Journal of biophotonics · 2025Article
- Article
- Sustainably cultured coral scaffold supports human bone marrow mesenchymal stromal cell osteogenesis.Regenerative therapy · 2024Article
- Microfluidically Aligned Collagen to Maintain the Phenotype of Tenocytes In Vitro.Advanced healthcare materials · 2024Article
- Liquefied capsules containing nanogrooved microdiscs and umbilical cord-derived cells for bone tissue engineering.Open research Europe · 2024Article
- Human Gingival Fibroblast and Osteoblast Behavior on Groove-Milled Zirconia Implant Surfaces.Materials (Basel, Switzerland) · 2022Article
- Effect of microtopography on osseointegration of implantable biomaterials and its modification strategies.Frontiers in bioengineering and biotechnology · 2022Review
- Effect of Controlled Microtopography on Osteogenic Differentiation of Mesenchymal Stem Cells.Journal of healthcare engineering · 2022Review
- Cell Behavior of Primary Fibroblasts and Osteoblasts on Plasma-Treated Fluorinated Polymer Coated with Honeycomb Polystyrene.Materials (Basel, Switzerland) · 2021Article
- Molecular Regulators of Cellular Mechanoadaptation at Cell-Material Interfaces.Frontiers in bioengineering and biotechnology · 2020Review
- Tailoring Cellular Function: The Contribution of the Nucleus in Mechanotransduction.Frontiers in bioengineering and biotechnology · 2020Review
- Microgrooves and Microrugosities in Titanium Implant Surfaces: An In Vitro and In Vivo Evaluation.Materials (Basel, Switzerland) · 2019Article
- Synthetic Biodegradable Aliphatic Polyester Nanocomposites Reinforced with Nanohydroxyapatite and/or Graphene Oxide for Bone Tissue Engineering Applications.Nanomaterials (Basel, Switzerland) · 2019Review
- The relationship between substrate topography and stem cell differentiation in the musculoskeletal system.Cellular and molecular life sciences : CMLS · 2019Review
- Effect of calcium glucoheptonate on proliferation and osteogenesis of osteoblast-like cells in vitro.PloS one · 2019Article
- The Evolution of Polystyrene as a Cell Culture Material.Tissue engineering. Part B, Reviews · 2018Review
- Square prism micropillars improve osteogenicity of poly(methyl methacrylate) surfaces.Journal of materials science. Materials in medicine · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Surface topography is increasingly being recognized as an important factor to control the response of cells and tissues to biomaterials. In the current study, the aim was to obtain deeper understanding of the effect of microgrooves on shape and orientation of osteoblast-like cells and to relate this effect to their proliferation and osteogenic differentiation. To this end, two microgrooved polystyrene (PS) substrates, differing in the width of the grooves (about 2 μm and 4 μm) and distance between individual grooves (about 6 μm and 11 μm, respectively) were fabricated using a combination of photolithography and hot embossing. MG-63 human osteosarcoma cells were cultured on these microgrooved surfaces, with unpatterned hot-embossed PS substrate as a control. Scanning electron- and fluorescence microscopy analyses showed that on patterned surfaces, the cells aligned along the microgrooves. The cells cultured on 4 μm-grooves / 11 μm-ridges surface showed a more pronounced alignment and a somewhat smaller cell area and cell perimeter as compared to cells cultured on surface with 2 μm-grooves / 6 μm-ridges or unpatterned PS. PrestoBlue analysis and quantification of DNA amounts suggested that microgrooves used in this experiment did not have a strong effect on cell metabolic activity or proliferation. However, cell differentiation towards the osteogenic lineage was significantly enhanced when MG-63 cells were cultured on the 2/6 substrate, as compared to the 4/11 substrate or unpatterned PS. This effect on osteogenic differentiation may be related to differences in cell spreading between the substrates.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.