ArticleNature structural & molecular biology2016
Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative.
Article in Nature structural & molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Emergence of transferable daptomycin resistance in Gram-positive bacteria.npj antimicrobials and resistance · 2025Article
- A mobile genetic element-derived primase-polymerase harbors multiple activities implicated in DNA replication and repair.Nucleic acids research · 2025Article
- Article
- A Multimodal Approach towards Genomic Identification of Protein Inhibitors of Uracil-DNA Glycosylase.Viruses · 2023Article
- Mobile genetic element-encoded putative DNA primases composed of A-family polymerase-SSB pairs.Frontiers in molecular biosciences · 2023Article
- Staphylococcal self-loading helicases couple the staircase mechanism with inter domain high flexibility.Nucleic acids research · 2022Article
- Genome-Scale Mutational Analysis of Cathode-OxidizingFrontiers in microbiology · 2022Article
- Viruses with U-DNA: New Avenues for Biotechnology.Viruses · 2021Review
- A thermostable DNA primase-polymerase from a mobile genetic element involved in defence against environmental DNA.Environmental microbiology · 2020Article
- A novel DNA primase-helicase pair encoded by SCCeLife · 2020Article
- The global distribution and evolutionary history of the pT26-2 archaeal plasmid family.Environmental microbiology · 2019Article
- Target highlights in CASP13: Experimental target structures through the eyes of their authors.Proteins · 2019Article
- Article
- Integrated mobile genetic elements in Thaumarchaeota.Environmental microbiology · 2019Article
- Mass spectrometry-based analysis of macromolecular complexes ofFEBS open bio · 2019Article
- Article
- Staphylococcal Plasmids, Transposable and Integrative Elements.Microbiology spectrum · 2018Review
- Mobile Genetic Elements Associated with Antimicrobial Resistance.Clinical microbiology reviews · 2018Review
- Crystal Structure of an Unusual Single-Stranded DNA-Binding Protein Encoded by Staphylococcal Cassette Chromosome Elements.Structure (London, England : 1993) · 2018Article
- Novel Families of Archaeo-Eukaryotic Primases Associated with Mobile Genetic Elements of Bacteria and Archaea.Journal of molecular biology · 2018Article
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Authors and funding
6 authors.
Funding
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is a public-health threat worldwide. Although the mobile genomic island responsible for this phenotype, staphylococcal cassette chromosome (SCC), has been thought to be nonreplicative, we predicted DNA-replication-related functions for some of the conserved proteins encoded by SCC. We show that one of these, Cch, is homologous to the self-loading initiator helicases of an unrelated family of genomic islands, that it is an active 3'-to-5' helicase and that the adjacent ORF encodes a single-stranded DNA-binding protein. Our 2.9-Å crystal structure of intact Cch shows that it forms a hexameric ring. Cch, like the archaeal and eukaryotic MCM-family replicative helicases, belongs to the pre-sensor II insert clade of AAA+ ATPases. Additionally, we found that SCC elements are part of a broader family of mobile elements, all of which encode a replication initiator upstream of their recombinases. Replication after excision would enhance the efficiency of horizontal gene transfer.
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