Evidence map›Paper›PMID 27571136›Full record

ArticleEndocrinology2016

A Convenient Method for Evaluating Epithelial Cell Proliferation in the Whole Mammary Glands of Female Mice.

Grace E Berryhill, Ingrid Brust-Mascher, Jill H Huynh, Thomas R Famula, Colin Reardon, Russell C Hovey

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Intramammary Labeling of Epithelial Cell Division.Journal of mammary gland biology and neoplasia · 2024
    Article
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Grace E BerryhillDepartment of Animal Science (G.E.B., J.H.H., T.R.F., R.C.H.) and University of California Davis Health Sciences District Advanced Imaging Facility, School of Veterinary Medicine, (I.B.-M., C.R.), University of California, Davis, California 95616-8521.
Ingrid Brust-MascherDepartment of Animal Science (G.E.B., J.H.H., T.R.F., R.C.H.) and University of California Davis Health Sciences District Advanced Imaging Facility, School of Veterinary Medicine, (I.B.-M., C.R.), University of California, Davis, California 95616-8521.
Jill H HuynhDepartment of Animal Science (G.E.B., J.H.H., T.R.F., R.C.H.) and University of California Davis Health Sciences District Advanced Imaging Facility, School of Veterinary Medicine, (I.B.-M., C.R.), University of California, Davis, California 95616-8521.
Thomas R FamulaDepartment of Animal Science (G.E.B., J.H.H., T.R.F., R.C.H.) and University of California Davis Health Sciences District Advanced Imaging Facility, School of Veterinary Medicine, (I.B.-M., C.R.), University of California, Davis, California 95616-8521.
Colin ReardonDepartment of Animal Science (G.E.B., J.H.H., T.R.F., R.C.H.) and University of California Davis Health Sciences District Advanced Imaging Facility, School of Veterinary Medicine, (I.B.-M., C.R.), University of California, Davis, California 95616-8521.
Russell C HoveyDepartment of Animal Science (G.E.B., J.H.H., T.R.F., R.C.H.) and University of California Davis Health Sciences District Advanced Imaging Facility, School of Veterinary Medicine, (I.B.-M., C.R.), University of California, Davis, California 95616-8521.

Funding

"Mechanisms of diet-induced, estrogen-independent mammary growth"F31CA189421 · NCI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI BERRYHILL, GRACE ELLEN · 2014 to 2015
$70k
NCI NIH HHS F31 CA189421
6 · The paper itself

Abstract

The mammary glands (MG) undergo rapid expansion of the ductal network during puberty in response to endocrine cues including the potent mitogenic effects of estrogen. The proliferation of mammary epithelial cells occurs in a spatially distinctive manner, where terminal end buds located at the ductal termini are the primary site of cell division. Here, we present a relatively high throughput approach to spatially assess epithelial cell proliferation in whole mouse MG using histochemical detection of 5-ethynyl-2'-deoxyuridine in conjunction with a standard curve-based data deconvolution technique to semiquantitatively measure proliferation via wide-field epifluorescent microscopy. This approach was validated against the "gold standard" of counting labeled nuclei from confocal images utilizing computer-assisted image analysis. Our method proved sensitive enough to describe the significant and spatially variable proliferative response to low-dose estrogen after 108 hours. This flexible method presents a timely and economical approach to obtaining spatial information regarding epithelial cell proliferation in the mouse MG.

Indexed as

Cell ProliferationAnimalsCell CountDeoxyuridineFemaleHumansMammary Glands, HumanMice, Inbred BALB C5-ethynyl-2'-deoxyuridineDeoxyuridine

Identifiers

PMID27571136
PMCPMC5045499

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.