ArticleSensors (Basel, Switzerland)2016
Label-Free Ag⁺ Detection by Enhancing DNA Sensitized Tb(3+) Luminescence.
Article in Sensors (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 14 citations in OpenAlex.
- Article
- DNAzyme sensors for detection of metal ions in the environment and imaging them in living cells.Current opinion in biotechnology · 2017Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, the effect of Ag⁺ on DNA sensitized Tb(3+) luminescence was studied initially using the Ag⁺-specific RNA-cleaving DNAzyme, Ag10c. While we expected to observe luminescence quenching by Ag⁺, a significant enhancement was produced. Based on this observation, simple DNA oligonucleotide homopolymers were used with systematically varied sequence and length. We discovered that both poly-G and poly-T DNA have a significant emission enhancement by Ag⁺, while the absolute intensity is stronger with the poly-G DNA, indicating that a G-quadruplex DNA is not required for this enhancement. Using the optimized length of the G7 DNA (an oligo constituted with seven guanines), Ag⁺ was measured with a detection limit of 57.6 nM. The signaling kinetics, G7 DNA conformation, and the binding affinity of Tb(3+) to the DNA in the presence or absence of Ag⁺ are also studied to reveal the mechanism of emission enhancement. This observation is useful not only for label-free detection of Ag⁺, but also interesting for the rational design of new biosensors using Tb(3+) luminescence.
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