ArticlePloS one2016
Practical Recommendations for the Use of the GeneSwitch Gal4 System to Knock-Down Genes in Drosophila melanogaster.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 39 citations in OpenAlex.
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- Engineering Protein Stability with Small Molecules: A Review of the ecDHFR Destabilizing Domain System.ACS chemical biology · 2026Review
- Light-inducible FLPase reconstitution enables temporal control of gene expression in Drosophila melanogaster.Cell reports methods · 2026Article
- High-fat diet impairs intermediate-term memory by autophagic-lysosomal dysfunction in Drosophila.PLoS genetics · 2025Article
- Repeat mediated excision of gene drive elements for restoring wild-type populations.PLoS genetics · 2024Article
- Pharmacology of Aging:Aging biology · 2024Article
- TransgenicCurrent issues in molecular biology · 2024Article
- The RU486-dependent activation of the GeneSwitch system in adult muscles leads to severe adverse effects in Drosophila.G3 (Bethesda, Md.) · 2024Article
- Repeat mediated excision of gene drive elements for restoring wild-type populations.bioRxiv : the preprint server for biology · 2023Article
- An Adapted GeneSwitch Toolkit for Comparable Cellular and Animal Models: A Proof of Concept in Modeling Charcot-Marie-Tooth Neuropathy.International journal of molecular sciences · 2023Article
- Analysis of asymptomatic Drosophila models for ALS and SMA reveals convergent impact on functional protein complexes linked to neuro-muscular degeneration.BMC genomics · 2023Article
- split-intein Gal4 provides intersectional genetic labeling that is repressible by Gal80.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Engineering Rapalog-Inducible Genetic Switches Based on Split-T7 Polymerase to Regulate Oncolytic Virus-Driven Production of Tumour-Localized IL-12 for Anti-Cancer Immunotherapy.Pharmaceuticals (Basel, Switzerland) · 2023Article
- split-intein Gal4 provides intersectional genetic labeling that is fully repressible by Gal80.bioRxiv : the preprint server for biology · 2023Article
- An optimized temporally controlled Gal4 system inFrontiers in neuroscience · 2023Article
- Uric acid metabolism modulates diet-dependent responses to intraspecific competition iniScience · 2022Article
- Transcriptional memory of dFOXO activation in youth curtails later-life mortality through chromatin remodeling and Xbp1.Nature aging · 2022Article
- Genetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes.Scientific reports · 2022Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Drosophila melanogaster is a popular research model organism thanks to its' powerful genetic tools that allow spatial and temporal control of gene expression. The inducible GeneSwitch Gal4 system (GS) system is a modified version of the classic UAS/GAL4 system which allows inducible regulation of gene expression and eliminates background effects. It is widely acknowledged that the GS system is leaky, with low level expression of UAS transgenes in absence of the inducer RU-486 (the progesterone analog that activates the modified GAL4 protein). However, in the course of our experiments, we have observed that the extent of this leak depends on the nature of the transgene being expressed. In the absence of RU-486, when strong drivers are used to express protein coding transgenes, leaky expression is low or negligible, however expression of RNA interference (RNAi) transgenes results in complete depletion of protein levels. The majority of published studies, using the GS system and RNAi transgenes validate knock-down efficiency by comparing target gene mRNA levels between induced and non-induced groups. Here, we demonstrate that this approach is lacking and that both additional control groups and further validation is required at the protein level. Unfortunately, this experimental limitation of the GS system eliminates "the background advantage", but does offer the possibility of performing more complex experiments (e.g. studying depletion and overexpression of different proteins in the same genetic background). The limitations and new possible applications of the GS system are discussed in detail.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.