ArticleBiomedical optics express2016
Incubator embedded cell culture imaging system (EmSight) based on Fourier ptychographic microscopy.
Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 55 citations in OpenAlex.
- Article
- Review
- Fast Fourier ptychographic quantitative phase microscopy for in vitro label-free imaging.Biomedical optics express · 2024Article
- Spatial- and Fourier-domain ptychography for high-throughput bio-imaging.Nature protocols · 2023Review
- Implementation of miniaturized modular-array fluorescence microscopy for long-term live-cell imaging.Applied optics · 2023Article
- Optical ptychography for biomedical imaging: recent progress and future directions [Invited].Biomedical optics express · 2023Review
- Implementation of free-space Fourier Ptychography with near maximum system numerical aperture.Optics express · 2022Article
- Article
- Cellular analysis using label-free parallel array microscopy with Fourier ptychography.Biomedical optics express · 2022Article
- All-in-focus fine needle aspiration biopsy imaging based on Fourier ptychographic microscopy.Journal of pathology informatics · 2022Article
- Picroscope: low-cost system for simultaneous longitudinal biological imaging.Communications biology · 2021Article
- PhaseRMiC: phase real-time microscope camera for live cell imaging.Biomedical optics express · 2021Article
- Miniaturized modular-array fluorescence microscopy.Biomedical optics express · 2020Article
- A versatile and customizable low-cost 3D-printed open standard for microscopic imaging.Nature communications · 2020Article
- Parallel Fourier ptychographic microscopy for high-throughput screening with 96 cameras (96 Eyes).Scientific reports · 2019Article
- Continuous Live-Cell Culture Imaging and Single-Cell Tracking by Computational Lensfree LED Microscopy.Sensors (Basel, Switzerland) · 2019Article
- Highly sensitive lens-free fluorescence imaging device enabled by a complementary combination of interference and absorption filters.Biomedical optics express · 2018Article
- Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression.Journal of visualized experiments : JoVE · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Multi-day tracking of cells in culture systems can provide valuable information in bioscience experiments. We report the development of a cell culture imaging system, named EmSight, which incorporates multiple compact Fourier ptychographic microscopes with a standard multiwell imaging plate. The system is housed in an incubator and presently incorporates six microscopes. By using the same low magnification objective lenses as the objective and the tube lens, the EmSight is configured as a 1:1 imaging system that, providing large field-of-view (FOV) imaging onto a low-cost CMOS imaging sensor. The EmSight improves the image resolution by capturing a series of images of the sample at varying illumination angles; the instrument reconstructs a higher-resolution image by using the iterative Fourier ptychographic algorithm. In addition to providing high-resolution brightfield and phase imaging, the EmSight is also capable of fluorescence imaging at the native resolution of the objectives. We characterized the system using a phase Siemens star target, and show four-fold improved coherent resolution (synthetic NA of 0.42) and a depth of field of 0.2 mm. To conduct live, long-term dopaminergic neuron imaging, we cultured ventral midbrain from mice driving eGFP from the tyrosine hydroxylase promoter. The EmSight system tracks movements of dopaminergic neurons over a 21 day period.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.