ArticleInternational journal of biological sciences2016
Selection of Reference Genes for Expression Studies of Xenobiotic Adaptation in Tetranychus urticae.
Article in International journal of biological sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 33 citations in OpenAlex.
- Complex Roles of Insect Cytochrome P450s in Chemical Adaptation.International journal of biological sciences · 2026Review
- Expression reduction and a variant of a P450 gene mediate chlorpyrifos resistance in Tetranychus urticae Koch.Journal of advanced research · 2025Article
- The Protective Effects of Pectic Polysaccharides on Dextran Sulfate Sodium-Induced Colitis inNutrients · 2025Article
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- Spider mite tetranins elicit different defense responses in different host habitats.The Plant journal : for cell and molecular biology · 2025Article
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- Molecular Mechanisms Underlying Metabolic Resistance to Cyflumetofen and Bifenthrin inInternational journal of molecular sciences · 2022Article
- Identification of Reliable Reference Genes under Different Stresses and in Different Tissues ofGenes · 2022Article
- Reference Genes for Expression Analyses by qRT-PCR inInsects · 2022Article
- Identification of suitable reference genes for expression profiling studies using qRT-PCR in an important insect pest, Maruca vitrata.Molecular biology reports · 2021Article
- Reference Gene Selection for Analyzing the Transcription Patterns of Two Fatty Acyl-CoA Reductase Genes From Paracoccus marginatus (Hemiptera: Pseudococcidae).Journal of insect science (Online) · 2021Article
- Article
- Reference gene validation in Eotetranychus sexmaculatus (Acari: Tetranychidae) feeding on mite-susceptible and mite-resistant rubber tree germplasms.Experimental & applied acarology · 2020Article
- Juvenile Spider Mites Induce Salicylate Defenses, but Not Jasmonate Defenses, Unlike Adults.Frontiers in plant science · 2020Article
- Evaluation and Validation of Reference Genes for Quantitative Real-Time PCR in Helopeltis theivora Waterhouse (Hemiptera: Miridae).Scientific reports · 2019Article
- Reference genes for gene expression studies by RT-qPCR in Brevipalpus yothersi (Acari: Tenuipalpidae), the mite vector of citrus leprosis virus.Scientific reports · 2019Article
- Screening potential reference genes for quantitative real-time PCR analysis in the oriental armyworm, Mythimna separata.PloS one · 2018Article
- HvPap-1 C1A Protease Participates Differentially in the Barley Response to a Pathogen and an Herbivore.Frontiers in plant science · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time PCR (qRT-PCR) is an extensively used, high-throughput method to analyze transcriptional expression of genes of interest. An appropriate normalization strategy with reliable reference genes is required for calculating gene expression across diverse experimental conditions. In this study, we aim to identify the most stable reference genes for expression studies of xenobiotic adaptation in Tetranychus urticae, an extremely polyphagous herbivore causing significant yield reduction of agriculture. We chose eight commonly used housekeeping genes as candidates. The qRT-PCR expression data for these genes were evaluated from seven populations: a susceptible and three acaricide resistant populations feeding on lima beans, and three other susceptible populations which had been shifted host from lima beans to three other plant species. The stability of the candidate reference genes was then assessed using four different algorithms (comparative ΔCt method, geNorm, NormFinder, and BestKeeper). Additionally, we used an online web-based tool (RefFinder) to assign an overall final rank for each candidate gene. Our study found that CycA and Rp49 are best for investigating gene expression in acaricide susceptible and resistant populations. GAPDH, Rp49, and Rpl18 are best for host plant shift studies. And GAPDH and Rp49 were the most stable reference genes when investigating gene expression under changes in both experimental conditions. These results will facilitate research in revealing molecular mechanisms underlying the xenobiotic adaptation of this notorious agricultural pest.
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