ArticleStem cell reports2016
Quantitative Analysis of Human Pluripotency and Neural Specification by In-Depth (Phospho)Proteomic Profiling.
Article in Stem cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Proteomic analyses in early brain development and neuropathological implications of fetal growth restriction.Neural regeneration research · 2026Article
- Macromolecular crowding in human tenocyte and skin fibroblast cultures: A comparative analysis.Materials today. Bio · 2024Article
- Histone Trimethylations and HDAC5 Regulate Spheroid Subpopulation and Differentiation Signaling of Human Adipose-Derived Stem Cells.Stem cells translational medicine · 2024Article
- Comprehensive Evaluation of Different TiOCells · 2022Article
- The promise of organoids for unraveling the proteomic landscape of the developing human brain.Molecular psychiatry · 2022Review
- Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells.Stem cell reports · 2021Article
- The quest of cell surface markers for stem cell therapy.Cellular and molecular life sciences : CMLS · 2021Review
- Chemical mutagenesis of a GPCR ligand: Detoxifying "inflammo-attraction" to direct therapeutic stem cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Dynamic Proteome of Oligodendrocyte Lineage Differentiation Features Planar Cell Polarity and Macroautophagy Pathways.GigaScience · 2020Article
- Targeted chromatin conformation analysis identifies novel distal neural enhancers of ZEB2 in pluripotent stem cell differentiation.Human molecular genetics · 2020Article
- Robotic High-Throughput Biomanufacturing and Functional Differentiation of Human Pluripotent Stem Cells.bioRxiv : the preprint server for biology · 2020Article
- A genetically defined disease model reveals that urothelial cells can initiate divergent bladder cancer phenotypes.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Systems-level Characterization of the Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells.Molecular & cellular proteomics : MCP · 2019Article
- NitroSynapsin for the treatment of neurological manifestations of tuberous sclerosis complex in a rodent model.Neurobiology of disease · 2019Article
- Chemically Defined Neural Conversion of Human Pluripotent Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Article
- The state of the art in stem cell biology and regenerative medicine: the end of the beginning.Pediatric research · 2018Review
- Spatiotemporal Proteomic Profiling of Human Cerebral Development.Molecular & cellular proteomics : MCP · 2017Article
- Corticosterone primes the neuroinflammatory response to Gulf War Illness-relevant organophosphates independently of acetylcholinesterase inhibition.Journal of neurochemistry · 2017Article
Corrections and comments
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Authors and funding
16 authors.
Funding
Abstract
Controlled differentiation of human embryonic stem cells (hESCs) can be utilized for precise analysis of cell type identities during early development. We established a highly efficient neural induction strategy and an improved analytical platform, and determined proteomic and phosphoproteomic profiles of hESCs and their specified multipotent neural stem cell derivatives (hNSCs). This quantitative dataset (nearly 13,000 proteins and 60,000 phosphorylation sites) provides unique molecular insights into pluripotency and neural lineage entry. Systems-level comparative analysis of proteins (e.g., transcription factors, epigenetic regulators, kinase families), phosphorylation sites, and numerous biological pathways allowed the identification of distinct signatures in pluripotent and multipotent cells. Furthermore, as predicted by the dataset, we functionally validated an autocrine/paracrine mechanism by demonstrating that the secreted protein midkine is a regulator of neural specification. This resource is freely available to the scientific community, including a searchable website, PluriProt.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.