ArticleJournal of biotechnology2016
Improving the extraction and purification of immunoglobulin G by the use of ionic liquids as adjuvants in aqueous biphasic systems.
Article in Journal of biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Towards Enhanced Tunability of Aqueous Biphasic Systems: Furthering the Grasp of Fluorinated Ionic Liquids in the Purification of Proteins.International journal of molecular sciences · 2024Article
- Combined Use of Ionic Liquid-Based Aqueous Biphasic Systems and Microfluidic Devices for the Detection of Prostate-Specific Antigen.Biosensors · 2023Article
- Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation.Frontiers in bioengineering and biotechnology · 2023Review
- Application of non-metal nanoparticles, as a novel approach, for improving the stability of blood products: 2011-2021.Progress in biomaterials · 2022Review
- Advances Brought by Hydrophilic Ionic Liquids in Fields Involving Pharmaceuticals.Materials (Basel, Switzerland) · 2021Review
- Understanding the Effect of Ionic Liquids as Adjuvants in the Partition of Biomolecules in Aqueous Two-Phase Systems Formed by Polymers and Weak Salting-Out Agents.Biochemical engineering journal · 2019Article
- Confinement of Suspension-Cultured Cells in Polyethylene Glycol/Polyethylene Oxide-Albumin Aqueous Two-Phase Systems.Frontiers in chemistry · 2019Article
- Separation of immunoglobulin G using aqueous biphasic systems composed of cholinium-based ionic liquids and poly(propylene glycol).Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) · 2018Article
- Evaluation of the effect of ionic liquids as adjuvants in polymer-based aqueous biphasic systems using biomolecules as molecular probes.Separation and purification technology · 2018Article
- Green Ultrasound versus Conventional Synthesis and Characterization of Specific Task Pyridinium Ionic Liquid Hydrazones Tethering Fluorinated Counter Anions: Novel Inhibitors of Fungal Ergosterol Biosynthesis.Molecules (Basel, Switzerland) · 2017Article
- Ultrasonic Assisted Extraction of Paclitaxel from Taxus x media Using Ionic Liquids as Adjuvants: Optimization of the Process by Response Surface Methodology.Molecules (Basel, Switzerland) · 2017Article
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Authors and funding
5 authors.
Funding
Abstract
Immunoglobulins G (IgG) could become widespread biopharmaceuticals if cost-efficient processes for their extraction and purification are available. In this work, aqueous biphasic systems (ABS) composed of polyethylene glycols and a buffered salt, and with ionic liquids (ILs) as adjuvants, have been studied as alternative extraction and purification platforms of IgG from a rabbit serum source. Eleven ILs were investigated to provide insights on the chemical features which maximize the IgG partitioning. It is shown that in polymer-salt systems pure IgG preferentially partitions to the polymer-rich phase; yet, the complete extraction was never attained. Remarkably, after the addition of 5wt% of adequate ILs to polymer-salt ABS, the complete extraction of pure IgG in a single-step was accomplished. The best systems and conditions were then applied to the extraction and purification of IgG directly from rabbit serum samples. The complete extraction of IgG in a single-step was maintained while its purity in the polymer-rich phase was enhanced by ca. 37% as compared to the IL-free ABS. The antibody stability was also evaluated revealing that appropriate ILs are able to maintain the IgG stability and can be used as phase-forming components of ABS when envisaging the purification of high-cost biopharmaceuticals.
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