ArticleGenome biology and evolution2016
Essentiality Is a Strong Determinant of Protein Rates of Evolution during Mutation Accumulation Experiments in Escherichia coli.
Article in Genome biology and evolution, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 29 citations in OpenAlex.
- Multiple molecular and cellular properties jointly affect protein and site-specific evolutionary rates.bioRxiv : the preprint server for biology · 2026Article
- Network architecture of transcriptomic stress responses in zebrafish embryos.PLoS computational biology · 2025Article
- A Short-Term View of Protein Sequence Evolution from Salmonella.Genome biology and evolution · 2025Article
- The overlooked evolutionary dynamics of 16S rRNA revises its role as the "gold standard" for bacterial species identification.Scientific reports · 2024Article
- Impact of transient acquired hypermutability on the inter- and intra-species competitiveness of Pseudomonas aeruginosa.The ISME journal · 2023Article
- Purifying selection enduringly acts on the sequence evolution of highly expressed proteins in Escherichia coli.G3 (Bethesda, Md.) · 2022Article
- Evolutionary coupling range varies widely among enzymes depending on selection pressure.Biophysical journal · 2021Article
- Avoidance of protein unfolding constrains protein stability in long-term evolution.Biophysical journal · 2021Article
- Universal Constraints on Protein Evolution in the Long-Term Evolution Experiment with Escherichia coli.Genome biology and evolution · 2021Article
- Richard Dickerson, Molecular Clocks, and Rates of Protein Evolution.Journal of molecular evolution · 2021Article
- Bacterial Protein Interaction Networks: Connectivity is Ruled by Gene Conservation, Essentiality and Function.Current genomics · 2021Article
- "Protein" no longer means what it used to.Current research in structural biology · 2021Review
- Evolution from Free-Living Bacteria to Endosymbionts of Insects: Genomic Changes and the Importance of the Chaperonin GroEL.Results and problems in cell differentiation · 2020Article
- A dispensable paralog of succinate dehydrogenase subunit C mediates standing resistance towards a subclass of SDHI fungicides in Zymoseptoria tritici.PLoS pathogens · 2019Article
- Molecular Chaperones Accelerate the Evolution of Their Protein Clients in Yeast.Genome biology and evolution · 2019Article
- Metabolic Determinants of Enzyme Evolution in a Genome-Scale Bacterial Metabolic Network.Genome biology and evolution · 2018Article
- Position Matters: Network Centrality Considerably Impacts Rates of Protein Evolution in the Human Protein-Protein Interaction Network.Genome biology and evolution · 2017Article
- Chance and necessity in the genome evolution of endosymbiotic bacteria of insects.The ISME journal · 2017Article
- Secreted Proteins Defy the Expression Level-Evolutionary Rate Anticorrelation.Molecular biology and evolution · 2017Article
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Authors and funding
5 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Neutral Theory of Molecular Evolution is considered the most powerful theory to understand the evolutionary behavior of proteins. One of the main predictions of this theory is that essential proteins should evolve slower than dispensable ones owing to increased selective constraints. Comparison of genomes of different species, however, has revealed only small differences between the rates of evolution of essential and nonessential proteins. In some analyses, these differences vanish once confounding factors are controlled for, whereas in other cases essentiality seems to have an independent, albeit small, effect. It has been argued that comparing relatively distant genomes may entail a number of limitations. For instance, many of the genes that are dispensable in controlled lab conditions may be essential in some of the conditions faced in nature. Moreover, essentiality can change during evolution, and rates of protein evolution are simultaneously shaped by a variety of factors, whose individual effects are difficult to isolate. Here, we conducted two parallel mutation accumulation experiments in Escherichia coli, during 5,500-5,750 generations, and compared the genomes at different points of the experiments. Our approach (a short-term experiment, under highly controlled conditions) enabled us to overcome many of the limitations of previous studies. We observed that essential proteins evolved substantially slower than nonessential ones during our experiments. Strikingly, rates of protein evolution were only moderately affected by expression level and protein length.
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