A Sensitive Peptide Nucleic Acid Probe Assay for Detection of BRAF V600 Mutations in Melanoma.

Tai-Long Chen, John Wen-Cheng Chang, Jia-Juan Hsieh, Hsin-Yi Cheng, Chiuan-Chian Chiou

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Article in Cancer genomics & proteomics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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1 citing paper in PubMed.

Heterogeneity and frequency of BRAF mutations in primary melanoma: Comparison between molecular methods and immunohistochemistry.Oncotarget · 2017
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Authors and funding

5 authors.

Tai-Long ChenGraduate Institute of Biomedical Sciences, Chang Gung University, Taoyuan, Taiwan, R.O.C.

John Wen-Cheng ChangDivision of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Taoyuan, Taiwan, R.O.C. College of Medicine, Chang Gung University, Taoyuan, Taiwan, R.O.C.

Jia-Juan HsiehDivision of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Taoyuan, Taiwan, R.O.C. College of Medicine, Chang Gung University, Taoyuan, Taiwan, R.O.C.

Hsin-Yi ChengDivision of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital, Taoyuan, Taiwan, R.O.C. College of Medicine, Chang Gung University, Taoyuan, Taiwan, R.O.C.

Chiuan-Chian ChiouGraduate Institute of Biomedical Sciences, Chang Gung University, Taoyuan, Taiwan, R.O.C. Department of Thoracic Medicine, Chang Gung Memorial Hospital, Taoyuan, Taiwan, R.O.C. Department of Medical Biotechnology and Laboratory Science, Chang Gung University, Taoyuan, Taiwan, R.O.C. ccchiou@mail.cgu.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mutated v-Raf murine sarcoma viral oncogene homolog B (BRAF) is an important biomarker for the prediction of therapeutic efficacy of several anticancer drugs. The detection of BRAF mutation faces two challenges: Firstly, there are multiple types of mutations, and secondly, tumor samples usually contain various amounts of wild-type, normal tissues. Here, we describe a newly established method for sensitive detection of multiple types of BRAF V600 mutations in excess wild-type background. The method introduced a fluorophore-tagged peptide nucleic acid (PNA) to serve as both polymerase chain reaction (PCR) clamp and sensor probe, which inhibited the amplification of wild-type templates during PCR and revealed multiple types of mutant signals during melting analysis. We demonstrated the design and optimization process of the method, and applied it in the detection of BRAF mutations in 49 melanoma samples. This PNA probe assay method detected three types of mutations in 17 samples, and was much more sensitive than conventional PCR plus Sanger sequencing.

Indexed as

MutationPeptide Nucleic AcidsCell Line, TumorCodonDNA Mutational AnalysisGenotypeHumansMelanomaPolymerase Chain ReactionProto-Oncogene Proteins B-rafSensitivity and SpecificityCodonPeptide Nucleic AcidsProto-Oncogene Proteins B-rafBRAF mutationsmelanomamutation detectionpeptide nucleic acid probe

Identifiers

PMID27566656

PMCPMC5070627

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.