ArticlePlant biotechnology journal2017
mlo-based powdery mildew resistance in hexaploid bread wheat generated by a non-transgenic TILLING approach.
Article in Plant biotechnology journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers, 1 of them a synthesis that pooled it.
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Who cites it
89 citing papers in PubMed, 1 synthesis or guideline pooled it, 212 citations in OpenAlex.
- Unravelling consensus genomic regions conferring stem-related traits in wheat via meta-QTL analysis.BMC plant biology · 2025Pooled it
- Endogenous RALF peptide function is required for powdery mildew host colonization.The New phytologist · 2026Article
- The role of MLO in powdery mildew susceptibility depends on a combination of functional specialization and subcellular localization.Plant physiology · 2026Article
- Two Aquaporins Mitigate Growth-Defence Trade-Offs by Facilitating COPlant biotechnology journal · 2026Article
- Advances in gene cloning and functional genomics approaches for wheat (Triticum aestivum L.) improvement.The plant genome · 2026Review
- From triangle to pyramid: Understanding host-pathogen-microniome-environment interplay for sustainable, enviromics-empowered management of plant diseases.Plant communications · 2026Review
- Beyond Resistance Genes: Silencing Susceptibility.International journal of molecular sciences · 2026Review
- Molecular Identification and RNA-Based Management of Fungal Plant Pathogens: From PCR to CRISPR/Cas9.International journal of molecular sciences · 2026Review
- Chromosomal deletions in banana somaclonal variants reveal negative regulators of immunity underlyingProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A novel MLO protein CsMLO4 plays an essential role in cucumber resistance to target leaf spot.Horticulture research · 2025Article
- A semi-dominant NLR allele regulates growth and disease resistance in wheat.Plant biotechnology journal · 2025Article
- Turning Susceptibility into Strength: A New Era of Durable Resistance in Plants Through Genome Editing.Plants (Basel, Switzerland) · 2025Review
- Genome-Wide Characterization Highlights Key Roles for Bread Wheat MLO Genes in Powdery Mildew and Abiotic Stresses.Rice (New York, N.Y.) · 2025Article
- Fine-mapping of sm6.1, a novel locus conferring resistance to gray leaf spot from Solanum habrochaites accession LA1777.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Silencing of disease susceptibility genes: an effective disease resistance strategy against fungal pathogens.Plant cell reports · 2025Review
- Gene editing of the E3 ligase PIRE1 fine-tunes reactive oxygen species production for enhanced bacterial disease resistance in tomato.The Plant cell · 2025Article
- Directed mutagenesis in fruit crops.3 Biotech · 2025Review
- Wheat Chromatin Remodeling Protein TaSWP73 Contributes to Compatible Wheat-Powdery Mildew Interaction.International journal of molecular sciences · 2025Article
- The E3 ubiquitin ligase TaGW2 facilitates TaSnRK1γ and TaVPS24 degradation to enhance stripe rust susceptibility in wheat.Plant biotechnology journal · 2025Article
- Beyond the genome: the role of functional markers in contemporary plant breeding.Frontiers in plant science · 2025Review
29 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Wheat is one of the most widely grown cereal crops in the world and is an important food grain source for humans. However, wheat yields can be reduced by many abiotic and biotic stress factors, including powdery mildew disease caused by Blumeria graminis f.sp. tritici (Bgt). Generating resistant varieties is thus a major effort in plant breeding. Here, we took advantage of the non-transgenic Targeting Induced Lesions IN Genomes (TILLING) technology to select partial loss-of-function alleles of TaMlo, the orthologue of the barley Mlo (Mildew resistance locus o) gene. Natural and induced loss-of-function alleles (mlo) of barley Mlo are known to confer durable broad-spectrum powdery mildew resistance, typically at the expense of pleiotropic phenotypes such as premature leaf senescence. We identified 16 missense mutations in the three wheat TaMlo homoeologues, TaMlo-A1, TaMlo-B1 and TaMlo-D1 that each lead to single amino acid exchanges. Using transient gene expression assays in barley single cells, we functionally analysed the different missense mutants and identified the most promising candidates affecting powdery mildew susceptibility. By stacking of selected mutant alleles we generated four independent lines with non-conservative mutations in each of the three TaMlo homoeologues. Homozygous triple mutant lines and surprisingly also some of the homozygous double mutant lines showed enhanced, yet incomplete, Bgt resistance without the occurrence of discernible pleiotropic phenotypes. These lines thus represent an important step towards the production of commercial non-transgenic, powdery mildew-resistant bread wheat varieties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.