Evidence map›Paper›PMID 27565868›Full record

ArticleAnimal biotechnology2016

Characterization of Growth and Reproduction Performance, Transgene Integration, Expression, and Transmission Patterns in Transgenic Pigs Produced by piggyBac Transposition-Mediated Gene Transfer.

Fang Zeng, Zicong Li, Gengyuan Cai, Wenchao Gao, Gelong Jiang, Dewu Liu, Johann Urschitz, Stefan Moisyadi, Zhenfang Wu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Fang Zenga National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.
Zicong Lia National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.
Gengyuan Caic Institute of Animal Science , Guangdong Academy of Agricultural Sciences , Guangzhou , China.
Wenchao Gaoa National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.
Gelong Jianga National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.
Dewu Liua National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.
Johann Urschitzd Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine , University of Hawaii at Manoa , Honolulu , Hawaii , USA.
Stefan Moisyadid Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine , University of Hawaii at Manoa , Honolulu , Hawaii , USA.
Zhenfang Wua National Engineering Research Center for Breeding Swine Industry, College of Animal Science , South China Agricultural University , Guangzhou , China.
South China Agricultural University · CNUniversity of Hawaiʻi at Mānoa · USGuangdong Academy of Agricultural Sciences · CN

Funding

RESEARCH DESIGN AND BIOSTATISTICS COREU54MD007584 · NIMHD · UNIVERSITY OF HAWAII AT MANOA · PI HEDGES, JERRIS ROBERT, MOKUAU, NOREEN · 2012 to 2018
$21.1M
Transgenic Mouse, ICSI and IVF CoreP20GM103457 · NIGMS · UNIVERSITY OF HAWAII AT MANOA · PI WARD, WILLIAM S · 2012 to 2018
$12.9M
NIGMS NIH HHS P20 GM103457NIMHD NIH HHS U54 MD007584
6 · The paper itself

Abstract

Previously we successfully produced a group of EGFP-expressing founder transgenic pigs by a newly developed efficient and simple pig transgenesis method based on cytoplasmic injection of piggyBac plasmids. In this study, we investigated the growth and reproduction performance and characterized the transgene insertion, transmission, and expression patterns in transgenic pigs generated by piggyBac transposition. Results showed that transgene has no injurious effect on the growth and reproduction of transgenic pigs. Multiple copies of monogenic EGFP transgene were inserted at noncoding sequences of host genome, and passed from founder transgenic pigs to their transgenic offspring in segregation or linkage manner. The EGFP transgene was ubiquitously expressed in transgenic pigs, and its expression intensity was associated with transgene copy number but not related to its promoter DNA methylation level. To the best of our knowledge, this is first study that fully described the growth and reproduction performance, transgene insertion, expression, and transmission profiles in transgenic pigs produced by piggyBac system. It not only demonstrates that piggyBac transposition-mediated gene transfer is an effective and favorable approach for pig transgenesis, but also provides scientific information for understanding the transgene insertion, expression and transmission patterns in transgenic animals produced by piggyBac transposition.

Indexed as

Animals, Genetically ModifiedGene Transfer TechniquesSwineAnimalsDNA MethylationGenetic VectorsOrgan SizeTransgenesCopy numbermethylationpiggyBactransgenic pigstransposition

Identifiers

PMID27565868
PMCPMC5025028
OpenAlexW2506334709

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.