ArticleReproductive biology and endocrinology : RB&E2016
17β-estradiol modifies human spermatozoa mitochondrial function in vitro.
Article in Reproductive biology and endocrinology : RB&E, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Effect of Capacitation on Proteomic Profile and Mitochondrial Parameters of Spermatozoa in Bulls.Journal of proteome research · 2025Article
- Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm-Oocyte Interaction.Journal of proteome research · 2023Article
- An Overview of the Health Effects of Bisphenol A from a One Health Perspective.Animals : an open access journal from MDPI · 2023Review
- Expression of estrogen receptors, PELP1, and SRC in human spermatozoa and their associations with semen quality.Human cell · 2023Article
- Oxidative Stress in Assisted Reproductive Techniques, with a Focus on an Underestimated Risk Factor.Current issues in molecular biology · 2023Review
- Male Infertility and Oxidative Stress: A Focus on the Underlying Mechanisms.Antioxidants (Basel, Switzerland) · 2022Review
- Advanced oxidative processes in the degradation of 17β-estradiol present on surface waters: kinetics, byproducts and ecotoxicity.Environmental science and pollution research international · 2020Article
- Increased expression of PELP1 in human sperm is correlated with decreased semen quality.Asian journal of andrologyArticle
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIt is assumed that spermatozoa are target cells for estrogens however, the mechanism of their action is not fully understood. The aim of this study was to investigate the influence of 17β-estradiol (E2) on the human spermatozoa mitochondrial function.
methodsThe effects on spermatozoa of E2 at final concentrations of 10(-10), 10(-8) and 10(-6) M were studied regarding the following phenomena: (1) kinetics of intracellular free calcium ions changes (using Fluo-3), (2) mitochondrial membrane potential ΔΨm (using JC-1 fluorochrome), (3) production of superoxide anion in mitochondria (using MitoSOX RED dye), (4) spermatozoa vitality (propidium iodide staining) and (5) phosphatidylserine membrane translocation (staining with annexin V marked with fluorescein).
resultsE2 initiated rapid (within a few seconds) dose dependent increase of intracellular free calcium ions concentration. E2 was changing the mitochondrial membrane potential: 10(-8) M initiated significant increase of percentage of high ΔΨm spermatozoa while the 10(-6) M induced significant decrease of high ΔΨm cells. In spermatozoa stimulated with E2 10(-6) M a significant increase of mitochondrial superoxide anion level was observed. 2 h incubation of spermatozoa with E2 did not alter cells vitality nor stimulated phosphatidylserine membrane translocation, for all three doses.
conclusions17β-estradiol affected the human spermatozoa mitochondrial function. E2 in low concentration improved while in high concentration might deteriorate mitochondrial function.
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