ArticleBiomaterials2016
Dental cell sheet biomimetic tooth bud model.
Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- Biopolymer-Based 3D Printing for Dental-Pulp Complex Tissue Regeneration: Innovations and Challenges.Molecules (Basel, Switzerland) · 2026Review
- Tooth-on-a-chip to engineer early dental epithelial-mesenchymal interaction.Materials today. Bio · 2025Article
- The comprehensive progress of tooth regeneration from the tooth development to tissue engineering and clinical application.Cell regeneration (London, England) · 2025Review
- Harnessing the dental cells derived from human induced pluripotent stem cells for hard tissue engineering.Journal of advanced research · 2024Article
- Recent Advances in Scaffolds for Guided Bone Regeneration.Biomimetics (Basel, Switzerland) · 2024Review
- Research progress of biomimetic materials in oral medicine.Journal of biological engineering · 2023Review
- A 3D-Bioprinted Functional Module Based on Decellularized Extracellular Matrix Bioink for Periodontal Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Bone morphogenetic protein 7 mediates stem cells migration and angiogenesis: therapeutic potential for endogenous pulp regeneration.International journal of oral science · 2022Article
- Synthetic materials in craniofacial regenerative medicine: A comprehensive overview.Frontiers in bioengineering and biotechnology · 2022Review
- 3D-bioprinted Recombination Structure of Hertwig's Epithelial Root Sheath Cells and Dental Papilla Cells for Alveolar Bone Regeneration.International journal of bioprinting · 2022Article
- Article
- Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration.International journal of molecular sciences · 2021Review
- Advanced in Vitro Experimental Models for Tissue Engineering-based Reconstruction of a 3D Dentin/pulp Complex: a Literature Review.Stem cell reviews and reports · 2021Review
- Taurine Augments Telomerase Activity and Promotes Chondrogenesis in Dental Pulp Stem Cells.Journal of personalized medicine · 2021Article
- Viability of Quercetin-Induced Dental Pulp Stem Cells in Forming Living Cellular Constructs for Soft Tissue Augmentation.Journal of personalized medicine · 2021Article
- Hard Dental Tissues Regeneration-Approaches and Challenges.Materials (Basel, Switzerland) · 2021Review
- The Role of Medicine and Technology in Shaping the Future of Oral Health.Journal of the California Dental Association · 2020Article
- Exosome-like vesicles derived from Hertwig's epithelial root sheath cells promote the regeneration of dentin-pulp tissue.Theranostics · 2020Article
- Tooth Bioengineering and Regenerative Dentistry.Journal of dental research · 2019Article
- Bioengineering Tooth Bud Constructs Using GelMA Hydrogel.Methods in molecular biology (Clifton, N.J.) · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Tissue engineering and regenerative medicine technologies offer promising therapies for both medicine and dentistry. Our long-term goal is to create functional biomimetic tooth buds for eventual tooth replacement in humans. Here, our objective was to create a biomimetic 3D tooth bud model consisting of dental epithelial (DE) - dental mesenchymal (DM) cell sheets (CSs) combined with biomimetic enamel organ and pulp organ layers created using GelMA hydrogels. Pig DE or DM cells seeded on temperature-responsive plates at various cell densities (0.02, 0.114 and 0.228 cells 10(6)/cm(2)) and cultured for 7, 14 and 21 days were used to generate DE and DM cell sheets, respectively. Dental CSs were combined with GelMA encapsulated DE and DM cell layers to form bioengineered 3D tooth buds. Biomimetic 3D tooth bud constructs were cultured in vitro, or implanted in vivo for 3 weeks. Analyses were performed using micro-CT, H&E staining, polarized light (Pol) microscopy, immunofluorescent (IF) and immunohistochemical (IHC) analyses. H&E, IHC and IF analyses showed that in vitro cultured multilayered DE-DM CSs expressed appropriate tooth marker expression patterns including SHH, BMP2, RUNX2, tenascin and syndecan, which normally direct DE-DM interactions, DM cell condensation, and dental cell differentiation. In vivo implanted 3D tooth bud constructs exhibited mineralized tissue formation of specified size and shape, and SHH, BMP2 and RUNX2and dental cell differentiation marker expression. We propose our biomimetic 3D tooth buds as models to study optimized DE-DM cell interactions leading to functional biomimetic replacement tooth formation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.