ArticleNature microbiology2016
Direct observation of rotation and steps of the archaellum in the swimming halophilic archaeon Halobacterium salinarum.
Article in Nature microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 97 citations in OpenAlex.
- Bacteria break through one-micrometer-square passages by flagellar wrapping.Nature communications · 2026Article
- Structure of a functional archaellum in Bacteria of the Chloroflexota phylum.Nature microbiology · 2025Article
- Species-specific surface dwell times and accumulation of microbes investigated by holographic microscopy.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2025Article
- Conformational changes in the motor ATPase CpaF facilitate a rotary mechanism of Tad pilus assembly.Nature communications · 2025Article
- How Does the Archaellum Work?Biomolecules · 2025Review
- Perturbed N-glycosylation of Halobacterium salinarum archaellum filaments leads to filament bundling and compromised cell motility.Nature communications · 2024Article
- Promising dawn in tumor microenvironment therapy: engineering oral bacteria.International journal of oral science · 2024Review
- N-glycosylation in Archaea - Expanding the process, components and roles of a universal post-translational modification.BBA advances · 2024Article
- VA-TIRFM-based SM kymograph analysis for dwell time and colocalization of plasma membrane protein in plant cells.Plant methods · 2023Article
- Flagellar polymorphism-dependent bacterial swimming motility in a structured environment.Biophysics and physicobiology · 2023Article
- Direct Observation of Archaellar Motor Rotation by Single-Molecular Imaging Techniques.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Comparative Genomic Insights into Chemoreceptor Diversity and Habitat Adaptation of Archaea.Applied and environmental microbiology · 2022Article
- Article
- An archaellum filament composed of two alternating subunits.Nature communications · 2022Article
- Towards Elucidating the Rotary Mechanism of the Archaellum Machinery.Frontiers in microbiology · 2022Review
- Methods to Analyze Motility in Eury- and Crenarchaea.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Article
- A comprehensive history of motility and Archaellation in Archaea.FEMS microbes · 2021Article
- How Did the Archaellum Get Its Rotation?Frontiers in microbiology · 2021Review
- Evolution of Archaellum Rotation Involved Invention of a Stator Complex by Duplicating and Modifying a Core Component.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Motile archaea swim using a rotary filament, the archaellum, a surface appendage that resembles bacterial flagella structurally, but is homologous to bacterial type IV pili. Little is known about the mechanism by which archaella produce motility. To gain insights into this mechanism, we characterized archaellar function in the model organism Halobacterium salinarum. Three-dimensional tracking of quantum dots enabled visualization of the left-handed corkscrewing of archaea in detail. An advanced analysis method combined with total internal reflection fluorescence microscopy, termed cross-kymography, was developed and revealed a right-handed helical structure of archaella with a rotation speed of 23 ± 5 Hz. Using these structural and kinetic parameters, we computationally reproduced the swimming and precession motion with a hydrodynamic model and estimated the archaellar motor torque to be 50 pN nm. Finally, in a tethered-cell assay, we observed intermittent pauses during rotation with ∼36° or 60° intervals, which we speculate may be a unitary step consuming a single adenosine triphosphate molecule, which supplies chemical energy of 80 pN nm when hydrolysed. From an estimate of the energy input as ten or six adenosine triphosphates per revolution, the efficiency of the motor is calculated to be ∼6-10%.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.