ArticlePLoS neglected tropical diseases2016
Clonal Evolution of Enterocytozoon bieneusi Populations in Swine and Genetic Differentiation in Subpopulations between Isolates from Swine and Humans.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Prevalence and Molecular Characterization of Enterocytozoon bieneusi in Horses From Northern and Eastern China: Investigation of Associated Risk Factors and Assessment of Zoonotic Potential.Transboundary and emerging diseases · 2026Article
- Identification of two new genetic loci for high-resolution genotyping of Enterocytozoon bieneusi.Parasite (Paris, France) · 2025Article
- Brown rats (Frontiers in veterinary science · 2024Article
- Intraspecific genetic diversity of the fish-infecting microsporidian parasiteFrontiers in microbiology · 2023Article
- Ecological and public health significance ofOne health (Amsterdam, Netherlands) · 2021Review
- Population genetic analysis suggests genetic recombination is responsible for increased zoonotic potential ofOne health (Amsterdam, Netherlands) · 2020Article
- Molecular detection of porcine Enterocytozoon bieneusi infection in Peninsular Malaysia and epidemiological risk factors associated with potentially zoonotic genotypes.Parasitology research · 2020Article
- Multilocus sequence typing of Enterocytozoon bieneusi in crab-eating macaques (Macaca fascicularis) in Hainan, China.Parasites & vectors · 2020Article
- Novel genotypes and multilocus genotypes of Enterocytozoon bieneusi in two wild rat species in China: potential for zoonotic transmission.Parasitology research · 2020Article
- Multilocus Sequence Typing ofFrontiers in microbiology · 2020Article
- Molecular detection of Enterocytozoon bieneusi in alpacas (Vicugna pacos) in Xinjiang, China.Parasite (Paris, France) · 2019Article
- Multilocus Sequence Typing and Population Genetic Analysis ofFrontiers in genetics · 2019Article
- Prevalence and Population Genetics Analysis ofFrontiers in microbiology · 2019Article
- First detection and genetic characterisation of Enterocytozoon bieneusi in wild deer in Melbourne's water catchments in Australia.Parasites & vectors · 2018Article
- Population genetics of Enterocytozoon bieneusi in captive giant pandas of China.Parasites & vectors · 2017Article
- High prevalence of Enterocytozoon bieneusi zoonotic genotype D in captive golden snub-nosed monkey (Rhinopithecus roxellanae) in zoos in China.BMC veterinary research · 2017Article
- Presence of zoonotic Cryptosporidium scrofarum, Giardia duodenalis assemblage A and Enterocytozoon bieneusi genotypes in captive Eurasian wild boars (Sus scrofa) in China: potential for zoonotic transmission.Parasites & vectors · 2017Article
- New Genotypes ofFrontiers in microbiology · 2017Article
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterocytozoon bieneusi is a widespread parasite with high genetic diversity among hosts. Its natural reservoir remains elusive and data on population structure are available only in isolates from primates. Here we describe a population genetic study of 101 E. bieneusi isolates from pigs using sequence analysis of the ribosomal internal transcribed spacer (ITS) and four mini- and microsatellite markers. The presence of strong linkage disequilibrium (LD) and limited genetic recombination indicated a clonal structure for the population. Bayesian inference of phylogeny, structural analysis, and principal coordinates analysis separated the overall population into three subpopulations (SP3 to SP5) with genetic segregation of the isolates at some geographic level. Comparative analysis showed the differentiation of SP3 to SP5 from the two known E. bieneusi subpopulations (SP1 and SP2) from primates. The placement of a human E. bieneusi isolate in pig subpopulation SP4 supported the zoonotic potential of some E. bieneusi isolates. Network analysis showed directed evolution of SP5 to SP3/SP4 and SP1 to SP2. The high LD and low number of inferred recombination events are consistent with the possibility of host adaptation in SP2, SP3, and SP4. In contrast, the reduced LD and high genetic diversity in SP1 and SP5 might be results of broad host range and adaptation to new host environment. The data provide evidence of the potential occurrence of host adaptation in some of E. bieneusi isolates that belong to the zoonotic ITS Group 1.
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