ArticleJournal of inflammation (London, England)2016
Acidification changes affect the inflammasome in human nucleus pulposus cells.
Article in Journal of inflammation (London, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 28 citations in OpenAlex.
- Trial
- Ginsenoside Rh2 Attenuates Intervertebral Disc Degeneration by Modulating the PI3K/AKT Signaling Pathway to Suppress Apoptosis.International journal of molecular sciences · 2026Article
- Feasibility Study on Inflammasome Proteins as Biomarkers in the Cerebrospinal Fluid of Pediatric Patients with Hydrocephalus Due to Intraventricular Hemorrhage.Biomolecules · 2025Article
- Human collagen III mRNA therapy for effective skin rejuvenation.Journal of translational medicine · 2025Article
- Lactylation: An Innovative Approach to Disease Control.Aging and disease · 2024Review
- Glutamine suppresses senescence and promotes autophagy through glycolysis inhibition-mediated AMPKα lactylation in intervertebral disc degeneration.Communications biology · 2024Article
- Regulatory Effect of Inflammatory Mediators in Intervertebral Disc Degeneration.Mediators of inflammation · 2023Review
- Role of Pyroptosis in Intervertebral Disc Degeneration and Its Therapeutic Implications.Biomolecules · 2022Review
- USP14 promotes pyroptosis of human annulus fibrosus cells derived from patients with intervertebral disc degeneration through deubiquitination of NLRP3.Acta biochimica et biophysica Sinica · 2022Article
- Renal and Inflammatory Proteins as Biomarkers of Diabetic Kidney Disease and Lupus Nephritis.Oxidative medicine and cellular longevity · 2022Article
- Acid-sensing ion channel 3: An analgesic target.Channels (Austin, Tex.) · 2021Review
- Tumor necrosis factor-alpha and interferon-gamma induce inflammasome-mediated corneal endothelial cell death.Experimental eye research · 2021Article
- Acid-sensing ion channels regulate nucleus pulposus cell inflammation and pyroptosis via the NLRP3 inflammasome in intervertebral disc degeneration.Cell proliferation · 2021Article
- Inflammasome Proteins as Inflammatory Biomarkers of Age-Related Macular Degeneration.Translational vision science & technology · 2020Article
- ASC, IL-18 and Galectin-3 as Biomarkers of Non-Alcoholic Steatohepatitis: A Proof of Concept Study.International journal of molecular sciences · 2020Article
- The Inflammasome Adaptor Protein ASC in Mild Cognitive Impairment and Alzheimer's Disease.International journal of molecular sciences · 2020Article
- Netosis and Inflammasomes in Large Vessel Occlusion Thrombi.Frontiers in pharmacology · 2020Article
- The Inflammasome Signaling Proteins ASC and IL-18 as Biomarkers of Psoriasis.Frontiers in pharmacology · 2020Article
- Article
- Defective Inflammatory Pathways in Never-Treated Depressed Patients Are Associated with Poor Treatment Response.Neuron · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInterleukin (IL)-1β is involved in the pathology of intervertebral disc degeneration. Under normal conditions, IL-1β is present in cells in an inactive form (pro-IL-1β). However, under pathological conditions, pro-IL-1β is turned into its active form (IL-1β) by the inflammasome, a multi-protein complex of the innate immune response that activates caspase-1. Under conditions of degeneration, the disc experiences an environment of increased acidification. However, the implications of acidification on the innate immune response remain poorly explored.
methodsHere we have studied how pH changes in human nucleus pulposus cells affect inflammasome activation by immunoblot analysis of protein lysates obtained from nucleus pulposus cells that were exposed to different pH levels in culture.
resultsIn this study, we have found that in nucleus pulposus cells, with increased acidification, there was a decrease in inflammasome activation consistent with lower levels of active IL-1β. However, this effect at a pH of 6.5, the lowest pH level tested, was abrogated when cells were treated with IL-1β.
conclusionsTaken together, these findings suggest that the inflammatory response through IL-1β experienced by the human disc is not initiated in nucleus pulposus cells when the stimulus is acidification.
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