Evidence map›Paper›PMID 27562917›Full record

ArticleArteriosclerosis, thrombosis, and vascular biology2016

Autotaxin-Lysophosphatidic Acid Axis Acts Downstream of Apoprotein B Lipoproteins in Endothelial Cells.

Liron Gibbs-Bar, Hanoch Tempelhof, Rotem Ben-Hamo, Yona Ely, Alexander Brandis, Roy Hofi, Gabriella Almog, Tslil Braun, Ester Feldmesser, Sol Efroni and 1 more

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Arteriosclerosis, thrombosis, and vascular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

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  7. Reporting Sex and Sex Differences in Preclinical Studies.Arteriosclerosis, thrombosis, and vascular biology · 2018
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Liron Gibbs-BarFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E).
Hanoch TempelhofFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E).
Rotem Ben-HamoFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E).
Yona ElyFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E).
Alexander BrandisFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E).
Roy HofiFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E).
Gabriella AlmogFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E).
Tslil BraunFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E).
Ester FeldmesserFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E).
Sol EfroniFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E).
Karina YanivFrom the Department of Biological Regulation (L.G.-B., H.T., Y.E., K.Y.), Department of Biological Services (E.F., A.B.), Department of Veterinary Services (R.H., G.A.), and Department of Molecular Genetics (T.B.), Weizmann Institute of Science, Rehovot, Israel; and Mina & Everard Goodman Faculty of Life Sciences, Bar Ilan University, Ramat Gan, Israel (R.B.-H., S.E). karina.yaniv@weizmann.ac.il.
Bar-Ilan University · IL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveAs they travel through the blood stream, plasma lipoproteins interact continuously with endothelial cells (ECs). Although the focus of research has mostly been guided by the importance of lipoproteins as risk factors for atherosclerosis, thrombosis, and other cardiovascular diseases, little is known about the mechanisms linking lipoproteins and angiogenesis under physiological conditions, and particularly, during embryonic development. In this work, we performed global mRNA expression profiling of endothelial cells from hypo-, and hyperlipidemic zebrafish embryos with the goal of uncovering novel mediators of lipoprotein signaling in the endothelium. APPROACH AND

resultsMicroarray analysis was conducted on fluorescence-activated cell sorting-isolated fli1:EGFP(+) ECs from normal, hypo-, and hyperlipidemic zebrafish embryos. We found that opposed levels of apoprotein B lipoproteins result in differential expression of the secreted enzyme autotaxin in ECs, which in turn affects EC sprouting and angiogenesis. We further demonstrate that the effects of autotaxin in vivo are mediated by lysophosphatidic acid (LPA)-a well-known autotaxin activity product-and that LPA and LPA receptors participate as well in the response of ECs to lipoprotein levels.

conclusionsOur findings provide the first in vivo gene expression profiling of ECs facing different levels of plasma apoprotein B lipoproteins and uncover a novel lipoprotein-autotaxin-LPA axis as regulator of EC behavior. These results highlight new roles for lipoproteins as signaling molecules, which are independent of their canonical function as cholesterol transporters.

Indexed as

Neovascularization, PhysiologicAnimalsAnimals, Genetically ModifiedApolipoproteins BCell ProliferationCells, CulturedDisease Models, AnimalEndothelial CellsGene Expression ProfilingGenotypeGreen Fluorescent ProteinsHumansHuman Umbilical Vein Endothelial CellsHyperlipidemiasLysophospholipase DLysophospholipidsApolipoproteins Benhanced green fluorescent proteinFli1b protein, zebrafishGreen Fluorescent Proteinslysophosphatidic acidLysophospholipase DLysophospholipidsPhosphoric Diester HydrolasesReceptors, Lysophosphatidic AcidTranscription FactorsZebrafish Proteinsautotaxincardiovascular diseaselipoproteinslysophosphatidic acidzebrafish

Identifiers

PMID27562917
OpenAlexW2507167490

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.