ArticlePsychopharmacology2016
Prevention of unpredictable chronic stress-related phenomena in zebrafish exposed to bromazepam, fluoxetine and nortriptyline.
Article in Psychopharmacology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic review and meta-analysis of 10 years of unpredictable chronic stress in zebrafish.Lab animal · 2023Pooled it
- Studying the Stress, Gene Expression, and Behavioral Changes Induced by Diazepam and Sertraline in a Zebrafish Model.Journal of applied toxicology : JAT · 2026Article
- A Standardized Unpredictable Chronic Stress Protocol for Adult Zebrafish.Current protocols · 2026Article
- Toxicity of pharmaceutical micropollutants on common carp (Cyprinus carpio) using blood biomarkers.Scientific reports · 2025Article
- Experimental PTSD Models in Zebrafish: A Systematic Review of Behavioral, Neurochemical, and Molecular Outcomes.Biology · 2025Review
- Exposure Effects of Environmentally Relevant Concentrations of the Tricyclic Antidepressant Amitriptyline in Early Life Stage Zebrafish.Environmental science & technology · 2024Article
- Acute clomipramine exposure elicits dose-dependent surfacing behavior in adult zebrafish (PeerJ · 2024Article
- Assessing Drug Administration Techniques in Zebrafish Models of Neurological Disease.International journal of molecular sciences · 2023Review
- Animal Inflammation-Based Models of Neuropsychiatric Disorders.Advances in experimental medicine and biology · 2023Article
- Multi-level toxicity assessment of the antidepressant venlafaxine in embryos/larvae and adults of zebrafish (Danio rerio).Genetics and molecular biology · 2023Article
- Evolutionarily conserved gene expression patterns for affective disorders revealed using cross-species brain transcriptomic analyses in humans, rats and zebrafish.Scientific reports · 2022Article
- Towards Modeling Anhedonia and Its Treatment in Zebrafish.The international journal of neuropsychopharmacology · 2022Article
- Zebrafish as a Tool in the Study of Sleep and Memory-related Disorders.Current neuropharmacology · 2022Review
- An Overview of Zebrafish Modeling Methods in Drug Discovery and Development.Advances in experimental medicine and biology · 2022Article
- Age-Dependent Effects of Chronic Stress on Zebrafish Behavior and Regeneration.Frontiers in physiology · 2022Article
- Baicalein prevents stress-induced anxiety behaviors in zebrafish model.Frontiers in pharmacology · 2022Article
- Zebrafish as an Animal Model for Testing Agents with Antidepressant Potential.Life (Basel, Switzerland) · 2021Review
- Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model.Scientific reports · 2021Article
- Production of bioactive recombinant human fibroblast growth factor 12 using a new transient expression vector in E. coli and its neuroprotective effects.Applied microbiology and biotechnology · 2021Article
- Extracellular matrix remodeling with stress and depression: Studies in human, rodent and zebrafish models.The European journal of neuroscience · 2021Review
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
rationaleSeveral model organisms have been employed to study the impacts of stress on biological systems. Different models of unpredictable chronic stress (UCS) have been established in rodents; however, these protocols are expensive, long-lasting, and require a large physical structure. Our group has recently reported an UCS protocol in zebrafish with several advantages compared to rodent models. We observed that UCS induced behavioral, biochemical, and molecular changes similar to those observed in depressed patients, supporting the translational relevance of the protocol.
objectivesConsidering that a pharmacological assessment is lacking in this zebrafish model, our aim was to evaluate the effects of anxiolytic (bromazepam) and antidepressant drugs (fluoxetine and nortriptyline) on behavioral (novel tank test), biochemical (whole-body cortisol), and molecular parameters (cox-2, tnf-α, il-6, and il-10 gene expression) in zebrafish subjected to UCS.
resultsWe replicated previous data showing that UCS induces behavioral and neuroendocrine alterations in zebrafish, and we show for the first time that anxiolytic and antidepressant drugs are able to prevent such effects. Furthermore, we extended the molecular characterization of the model, revealing that UCS increases expression of the pro-inflammatory markers cox-2 and il-6, which was also prevented by the drugs tested.
conclusionsThis study reinforces the use of zebrafish as a model organism to study the behavioral and physiological effects of stress. The UCS protocol may also serve as a screening tool for evaluating new drugs that can be used to treat psychiatric disorders with stress-related etiologies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.