The feather-degrading bacterial community in two soils as revealed by a specific primer targeting serine-type keratinolytic proteases.

Zhenhong Gu, Honghui Zhu, Xiaolin Xie, Yonghong Wang, Xiaodi Liu, Qing Yao

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Article in World journal of microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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5 · Who and what money

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6 authors at 2 institutions in 1 country.

Zhenhong GuCollege of Horticulture, Guangdong Engineering Research Center for Litchi, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Wushan St. 483, Tianhe Dist., Guangzhou, 510642, China.

Honghui ZhuGuangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology (Ministry-Guangdong Province Jointly Breeding Base) South China, Guangzhou, 510070, China. zhuhh@gdim.cn.

Xiaolin XieCollege of Horticulture, Guangdong Engineering Research Center for Litchi, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Wushan St. 483, Tianhe Dist., Guangzhou, 510642, China.

Yonghong WangGuangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, State Key Laboratory of Applied Microbiology (Ministry-Guangdong Province Jointly Breeding Base) South China, Guangzhou, 510070, China.

Xiaodi LiuCollege of Horticulture, Guangdong Engineering Research Center for Litchi, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Wushan St. 483, Tianhe Dist., Guangzhou, 510642, China.

Qing YaoCollege of Horticulture, Guangdong Engineering Research Center for Litchi, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Wushan St. 483, Tianhe Dist., Guangzhou, 510642, China. yaoqscau@scau.edu.cn.

South China Agricultural University · CNInstitute of Microbiology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Feather waste represents a huge resource of protein, but is underutilized due to its recalcitrant nature. Feather-degrading bacteria can biologically degrade feathers and have great potential for industries. In this study, we first designed a primer set (BC) suitable for exploring the diversity of the keratinolytic bacterial community with denatured gradient gel electrophoresis (DGGE). With the BC primer set, the difference in the keratinolytic bacterial community between a feather-dumping (FD) soil and a non feather-dumping (NFD) soil and the influence of feather addition (enrichment culture) on the keratinolytic bacterial community were investigated. DGGE and sequencing showed that keratinolytic bacteria in these soils belong to 2 phyla (Actinobacteria and Proteobacteria) and 9 genera (Micromonospora, Verrucosispora, Actinopolymorpha, Knoellia, Hyalangium, Stigmatella, Archangium, Cystobacter, and Luteimonas). Feather addition decreased the species richness of the keratinolytic bacteria in FD soil, but greatly increased the diversity, species richness and abundance in NFD soil. Moreover, feather addition to NFD soil induced some keratinolytic bacteria that were absent in all of the other soils. Collectively, these data indicate that keratinolytic bacteria are diverse in both FD and NFD soil, and some novel keratinolytic bacteria taxa might be revealed by using the BC primer set.

Indexed as

AnimalsBacteriaBacterial ProteinsBiodegradation, EnvironmentalDNA PrimersFeathersIndustrial MicrobiologyPhylogenyPoultrySerine ProteasesSoil MicrobiologyWaste ProductsBacterial ProteinsDNA PrimersSerine ProteasesWaste ProductsFeather additionFeather-degrading bacterial communityPCR-DGGESerine-type keratinolytic proteasesSpecific primer design

Identifiers

PMID27562599

DOI10.1007/s11274-016-2125-1

OpenAlexW2515570828

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.