ArticleNature communications2016
Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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Who cites it
104 citing papers in PubMed.
- Closed-Loop Optogenetic Control in a Microplate Reader.ACS synthetic biology · 2026Article
- A control theoretical approach to gene regulation reveals quantitative constraints for dynamic homeostasis in stochastic gene expression.Biochimica et biophysica acta. Molecular basis of disease · 2026Article
- Overriding Bioprocess Perturbations With a Cell-Machine Interface for Reliable Microbial Stress-Response Control.Microbial biotechnology · 2026Article
- Signal control during tissue regeneration in adult animals.Nature reviews. Molecular cell biology · 2026Review
- Article
- Resolving emergent transient oscillations in gene circuits with a growth-coupled model.Science advances · 2026Article
- Single-cell analysis and control of microbial systems using optogenetics.Current opinion in microbiology · 2026Review
- Dynamic Robust Control of Microbial Communities Using Cybergenetics.Methods in molecular biology (Clifton, N.J.) · 2026Article
- A stationary phase-specific bacterial green light sensor for enhancing metabolite production.Nature communications · 2025Article
- Closed-loop optogenetic control of cell biology enables outcome-driven microscopy.Nature communications · 2025Article
- Balancing Doses of EL222 and Light Improves Optogenetic Induction of Protein Production in Komagataella phaffii.Biotechnology and bioengineering · 2025Article
- Potent optogenetic regulation of gene expression in mammalian cells for bioproduction and basic research.Nucleic acids research · 2025Article
- A dual light-controlled co-culture system enables the regulation of population composition.Synthetic and systems biotechnology · 2025Article
- Materials and device strategies to enhance spatiotemporal resolution in bioelectronics.Nature reviews. Materials · 2025Article
- Optogenetic control of horizontally acquired genes prevent stuck fermentations in yeast.Microbiology spectrum · 2025Article
- Article
- Biotechnology systems engineering: preparing the next generation of bioengineers.Frontiers in systems biology · 2025Article
- Article
- Yeast cell responses and survival during periodic osmotic stress are controlled by glucose availability.eLife · 2024Article
- Deep model predictive control of gene expression in thousands of single cells.Nature communications · 2024Article
44 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dynamic control of gene expression can have far-reaching implications for biotechnological applications and biological discovery. Thanks to the advantages of light, optogenetics has emerged as an ideal technology for this task. Current state-of-the-art methods for optical expression control fail to combine precision with repeatability and cannot withstand changing operating culture conditions. Here, we present a novel fully automatic experimental platform for the robust and precise long-term optogenetic regulation of protein production in liquid Escherichia coli cultures. Using a computer-controlled light-responsive two-component system, we accurately track prescribed dynamic green fluorescent protein expression profiles through the application of feedback control, and show that the system adapts to global perturbations such as nutrient and temperature changes. We demonstrate the efficacy and potential utility of our approach by placing a key metabolic enzyme under optogenetic control, thus enabling dynamic regulation of the culture growth rate with potential applications in bacterial physiology studies and biotechnology.
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