ArticleParasites & vectors2016
The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Bacterial reprogramming of tick metabolism impacts vector fitness and susceptibility to infection.Nature microbiology · 2024Article
- A simple non-invasive method to collect soft tick saliva reveals differences inFrontiers in cellular and infection microbiology · 2023Article
- Low NETosis Induced inVaccines · 2022Article
- Rickettsial Pathogen Perturbs Tick Circadian Gene to Infect the Vertebrate Host.International journal of molecular sciences · 2022Article
- Evaluation of an Indirect Immunofluorescence Assay for the Detection ofMicroorganisms · 2022Article
- Article
- Redox Imbalance and Its Metabolic Consequences in Tick-Borne Diseases.Frontiers in cellular and infection microbiology · 2022Review
- Epidemiology, Diagnosis, and Control of Canine Infectious Cyclic Thrombocytopenia and Granulocytic Anaplasmosis: Emerging Diseases of Veterinary and Public Health Significance.Veterinary sciences · 2021Review
- Diseases Transmitted by the Black-Legged Ticks in the United States: A Comprehensive Review of the Literature.Cureus · 2021Review
- The genus Anaplasma: drawing back the curtain on tick-pathogen interactions.Pathogens and disease · 2021Review
- Comparative analysis ofHeliyon · 2021Article
- Review
- Rickettsial pathogen uses arthropod tryptophan pathway metabolites to evade reactive oxygen species in tick cells.Cellular microbiology · 2020Article
- Tick and Host Derived Compounds Detected in the Cement Complex Substance.Biomolecules · 2020Article
- Article
- The redox metabolic pathways function to limit Anaplasma phagocytophilum infection and multiplication while preserving fitness in tick vector cells.Scientific reports · 2019Article
- Polymicrobial Nature of Tick-Borne Diseases.mBio · 2019Article
- Modeling Modulation of the Tick Regulome in Response toFrontiers in physiology · 2019Article
- A reverse vaccinology approach to the identification and characterization of Ctenocephalides felis candidate protective antigens for the control of cat flea infestations.Parasites & vectors · 2018Article
- Ticks elicit variable fibrinogenolytic activities upon feeding on hosts with different immune backgrounds.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe intracellular bacteria Anaplasma phagocytophilum are emerging zoonotic pathogens affecting human and animal health, and a good model for the study of tick-host-pathogen interactions. This tick-borne pathogen is transmitted by Ixodes scapularis in the United States where it causes human granulocytic anaplasmosis. Tick midguts and salivary glands play a major role during tick feeding and development, and in pathogen acquisition, multiplication and transmission. Vertebrate host proteins are found in tick midguts after feeding and have been described in the salivary glands of fed and unfed ticks, suggesting a role for these proteins during tick feeding and development. Furthermore, recent results suggested the hypothesis that pathogen infection affects tick metabolic processes to modify host protein digestion and persistence in the tick with possible implications for tick physiology and pathogen life-cycle.
methodsTo address this hypothesis, herein we used I. scapularis female ticks fed on uninfected and A. phagocytophilum-infected sheep to characterize host protein content in midguts and salivary glands by proteomic analysis of tick tissues.
resultsThe results evidenced a clear difference in the host protein content between tick midguts and salivary glands in response to infection suggesting that A. phagocytophilum selectively manipulates the levels of vertebrate host proteins in ticks in a tissue-specific manner to facilitate pathogen infection, multiplication and transmission while preserving tick feeding and development. The mechanisms by which A. phagocytophilum manipulates the levels of vertebrate host proteins are not known, but the results obtained here suggested that it might include the modification of proteolytic pathways.
conclusionsThe results of this study provided evidence to support that A. phagocytophilum affect tick proteolytic pathways to selectively manipulate the levels of vertebrate host proteins in a tissue-specific manner to increase tick vector capacity. Investigating the biological relevance of host proteins in tick biology and pathogen infection and the mechanisms used by A. phagocytophilum to manipulate host protein content is essential to advance our knowledge of tick-host-pathogen molecular interactions. These results have implications for the identification of new targets for the development of vaccines for the control of tick-borne diseases.
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