ArticleIn vitro cellular & developmental biology. Animal2017
Establishment of primary bovine intestinal epithelial cell culture and clone method.
Article in In vitro cellular & developmental biology. Animal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Next-generation non-animal models for inflammatory bowel disease:NAM journal · 2025Review
- Novel and effective screening system for recombinant protein production in CHO cells.Scientific reports · 2024Article
- Modeling Paratuberculosis in Laboratory Animals, Cells, or Tissues: A Focus on Their Applications for Pathogenesis, Diagnosis, Vaccines, and Therapy Studies.Animals : an open access journal from MDPI · 2023Review
- Article
- Monoammonium glycyrrhizinate improves antioxidant capacity of calf intestinal epithelial cells exposed to heat stress in vitro.Journal of animal science · 2023Article
- Establishment and characterization of an immortalized bovine intestinal epithelial cell line.Journal of animal science · 2023Article
- Unraveling the pros and cons of various in vitro methodologies for ruminant nutrition: a review.Translational animal science · 2022Review
- Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to HMolecules (Basel, Switzerland) · 2022Article
- Robust Three-Dimensional (3D) Expansion of Bovine Intestinal Organoids: An In Vitro Model as a Potential Alternative to an In Vivo System.Animals : an open access journal from MDPI · 2021Article
- Advances in fatty acids nutrition in dairy cows: from gut to cells and effects on performance.Journal of animal science and biotechnology · 2020Review
- Establishment and identification of primary bovine omasal epithelial cells.In vitro cellular & developmental biology. Animal · 2020Article
- Transcriptomic Analyses Reveal the Protective Immune Regulation of Conjugated Linoleic Acids in Sheep Ruminal Epithelial Cells.Frontiers in physiology · 2020Article
- Isolation, culture, and identification of duck intestinal epithelial cells and oxidative stress model constructed.In vitro cellular & developmental biology. Animal · 2019Article
- Development and biochemical and immunological characterization of early passage and immortalized bovine intestinal epithelial cell lines from the ileum of a young calf.Cytotechnology · 2019Article
- Effects and immune responses of probiotic treatment in ruminants.Veterinary immunology and immunopathology · 2019Review
- Short-Chain Fatty Acids Regulate the Immune Responses via G Protein-Coupled Receptor 41 in Bovine Rumen Epithelial Cells.Frontiers in immunology · 2019Article
- Development of in vitro enteroids derived from bovine small intestinal crypts.Veterinary research · 2018Article
- Immunobiotics for the Bovine Host: Their Interaction with Intestinal Epithelial Cells and Their Effect on Antiviral Immunity.Frontiers in immunology · 2018Review
- Establishment of immortalized mouse intestinal epithelial cells line and study of effects of Arg-Arg on inflammatory response.In vitro cellular & developmental biology. Animal · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to establish bovine intestinal epithelial cell (BIEC) line and provide a novel clone cell method. Although various strategies of bovine cell culture and clone techniques have been reported, these methods remain not established. Here, we culture successfully primary BIECs and establish a novel clone cell method. Our result showed that BIECs could be successfully cultured and passaged about generation 5. These cellular aggregates and clusters were adherent loosely at day 2 of culture. Cell aggregates and clusters start to proliferate after approximately 4 d. The BIECs showed positive reaction against cytokeratin 18, E-cadherin, and characteristics of epithelial-like morphology. In addition, the fatty acid-binding proteins (FABPs), villin, and intestinal peptidase (IP) band were positive in BIECs. Our results suggest that the establishment of culturing and clone BIEC methods will apply to isolate and clone other primary cells. These BIECs could therefore contribute to the study of bovine intestinal nutrient absorption and regulation, immune regulation, and the pathogenesis of the bovine intestinal disease, which will provide intestinal cell model in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.