ArticlePlant molecular biology2016
D27E mutation of VTC1 impairs the interaction with CSN5B and enhances ascorbic acid biosynthesis and seedling growth in Arabidopsis.
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
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- The Regulation of ROS and Phytohormones in Balancing Crop Yield and Salt Tolerance.Antioxidants (Basel, Switzerland) · 2025Review
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Ascorbic acid-mediated reactive oxygen species homeostasis modulates the switch from tapetal cell division to cell differentiation in Arabidopsis.The Plant cell · 2023Article
- Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.Frontiers in plant science · 2023Review
- Kiwifruit MYBS1-like and GBF3 transcription factors influence l-ascorbic acid biosynthesis by activating transcription of GDP-L-galactose phosphorylase 3.The New phytologist · 2022Article
- Crystal Structures ofFrontiers in plant science · 2022Article
- Comparative Transcriptome Analysis Revealed the Key Genes Regulating Ascorbic Acid Synthesis inInternational journal of molecular sciences · 2021Article
- The role of GDP-l-galactose phosphorylase in the control of ascorbate biosynthesis.Plant physiology · 2021Article
- The mLife science alliance · 2019Article
- Modulation of Ethylene and Ascorbic Acid on Reactive Oxygen Species Scavenging in Plant Salt Response.Frontiers in plant science · 2019Review
- Redox Changes During the Cell Cycle in the Embryonic Root Meristem of Arabidopsis thaliana.Antioxidants & redox signaling · 2017Article
- Genetic Control of Ascorbic Acid Biosynthesis and Recycling in Horticultural Crops.Frontiers in chemistry · 2017Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our previous investigation revealed that GDP-Man pyrophosphorylase (VTC1), a vital ascorbic acid (AsA) biosynthesis enzyme, could be degraded through interaction with the photomorphogenic factor COP9 signalosome subunit 5B (CSN5B) in the darkness, demonstrating the posttranscriptional regulation of light signal in AsA production. Here, we further report that a point mutation in D27E of VTC1 disables the interaction with CSN5B, resulting in enhancement of AsA biosynthesis and seedling growth in Arabidopsis thaliana. To identify the interaction sites with CSN5B, we first predicted the key amino acids in VTC1 via bioinformatics analysis. And then we biochemically and genetically demonstrated that the 27th Asp was the amino acid that influenced the interaction of VTC1 with CSN5B in plants. Moreover, transgenic lines overexpressing the site-specific mutagenesis from D27 (Asp) into E27 (Glu) in VTC1 showed enhanced AsA accumulation and reduced H
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