ArticleScientific reports2016
Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
- Butyrate, IL-4, and EP4 Receptors: A Triad of Colorectal Homeostasis, Protecting Against Onset of Cancer and IBD?BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Assessing the health of the gut microbial organ: why and how?The Journal of clinical investigation · 2025Article
- Long-term exposure to constant light disrupts intestinal stem cells through sympathoexcitation-induced Wnt5a signaling inhibition.Gastroenterology report · 2025Article
- Immunomodulation aspects of gut microbiome-related interventional strategies in colorectal cancer.Medical oncology (Northwood, London, England) · 2024Review
- Nutrigenomic underpinnings of intestinal stem cells in inflammatory bowel disease and colorectal cancer development.Frontiers in genetics · 2024Review
- Characterization of Wnt signaling pathway under treatment of Lactobacillus acidophilus postbiotic in colorectal cancer using an integrated in silico and in vitro analysis.Scientific reports · 2023Article
- Comparative efficacy of a novel Bacillus subtilis-based probiotic and pharmacological zinc oxide on growth performance and gut responses in nursery pigs.Scientific reports · 2023Article
- Gut microbiota-stem cell niche crosstalk: A new territory for maintaining intestinal homeostasis.iMeta · 2022Review
- Conflicting Effects of Wnt-5a in Ulcerative Colitis: You Wnt Some, You Lose Some.Digestive diseases and sciences · 2022Article
- Investigation on the Inhibitory Effect of Wnt-5a on Colonic Mucosal Inflammation in Patients with Ulcerative Colitis.Digestive diseases and sciences · 2022Article
- New insights into natural products that target the gut microbiota: Effects on the prevention and treatment of colorectal cancer.Frontiers in pharmacology · 2022Review
- Review
- Neonatal necrotizing enterocolitis-associated DNA methylation signatures in the colon are evident in stool samples of affected individuals.Epigenomics · 2021Article
- Immune System, Microbiota, and Microbial Metabolites: The Unresolved Triad in Colorectal Cancer Microenvironment.Frontiers in immunology · 2021Review
- Intestinal enteroids recapitulate the effects of short-chain fatty acids on the intestinal epithelium.PloS one · 2020Article
- Pathobiont release from dysbiotic gut microbiota biofilms in intestinal inflammatory diseases: a role for iron?Journal of biomedical science · 2019Review
- Advances in Gut Microbiota of Viral Hepatitis Cirrhosis.BioMed research international · 2019Review
- Ingestion of Non-digestible Carbohydrates From Plant-Source Foods and Decreased Risk of Colorectal Cancer: A Review on the Biological Effects and the Mechanisms of Action.Frontiers in nutrition · 2019Review
- Dysbiosis of gut microbiota in promoting the development of colorectal cancer.Gastroenterology report · 2018Review
- The Research Progress on Intestinal Stem Cells and Its Relationship with Intestinal Microbiota.Frontiers in immunology · 2017Review
Corrections and comments
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Authors and funding
14 authors at 4 institutions in 3 countries.
Funding
Abstract
Proliferation and spatial development of colonic epithelial cells are highly regulated along the crypt vertical axis, which, when perturbed, can result in aberrant growth and carcinogenesis. In this study, two key factors were identified that have important and counterbalancing roles regulating these processes: pericrypt myofibroblast-derived Wnt-5a and the microbial metabolite butyrate. Cultured YAMC cell proliferation and heat shock protein induction were analzyed after butryate, conditioned medium with Wnt5a activity, and FrzB containing conditioned medium. In vivo studies to modulate Hsp25 employed intra-colonic wall Hsp25 encoding lentivirus. To silence Wnt-5a in vivo, intra-colonic wall Wnt-5a silencing RNA was used. Wnt-5a, secreted by stromal myofibroblasts of the lower crypt, promotes proliferation through canonical β-catenin activation. Essential to this are two key requirements: (1) proteolytic conversion of the highly insoluble ~40 kD Wnt-5a protein to a soluble 36 mer amino acid peptide that activates epithelial β-catenin and cellular proliferation, and (2) the simultaneous inhibition of butyrate-induced Hsp25 by Wnt-5a which is necessary to arrest the proliferative process in the upper colonic crypt. The interplay and spatial gradients of these factors insures that crypt epithelial cell proliferation and development proceed in an orderly fashion, but with sufficient plasticity to adapt to physiological perturbations including inflammation.
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Registered trials
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