Evidence map›Paper›PMID 27559438›Full record

ArticleAsian spine journal2016

Transplantation of Neural Stem Cells Cultured in Alginate Scaffold for Spinal Cord Injury in Rats.

Seyed Mojtaba Hosseini, Ali Sharafkhah, Omid Koohi-Hosseinabadi, Maryam Semsar-Kazerooni

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Asian spine journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 3 pooled it
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 3 syntheses or guidelines pooled it, 39 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Alginate scaffolds improve functional recovery after spinal cord injury.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2022
    Pooled it
  4. Article
  5. Review
  6. The Application of Biomaterials in Spinal Cord Injury.International journal of molecular sciences · 2023
    Review
  7. Article
  8. Manganese-Labeled Alginate Hydrogels for Image-Guided Cell Transplantation.International journal of molecular sciences · 2022
    Article
  9. Cell transplantation to repair the injured spinal cord.International review of neurobiology · 2022
    Article
  10. A State-of-the-Art of Functional Scaffolds for 3D Nervous Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2021
    Review
  11. Article
  12. Article
  13. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Seyed Mojtaba HosseiniStudent Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.; Cell and Molecular Medicine Student Research Group, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.; Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.; Stem Cell Laboratory, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran.
Ali SharafkhahStudent Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.; Cell and Molecular Medicine Student Research Group, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.
Omid Koohi-HosseinabadiStudent Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.; Cell and Molecular Medicine Student Research Group, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.; Laboratory Animals Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Maryam Semsar-KazerooniStudent Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.; Cell and Molecular Medicine Student Research Group, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.
Shiraz University of Medical Sciences · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

STUDY

designThis study investigated the effects of transplantation of alginate encapsulated neural stem cells (NSCs) on spinal cord injury in Sprague-Dawley male rats. The neurological functions were assessed for 6 weeks after transplantation along with a histological study and measurement of caspase-3 levels. PURPOSE: The aim of this study was to discover whether NSCs cultured in alginate transplantation improve recovery from spinal cord injury. OVERVIEW OF LITERATURE: Spinal cord injury is one of the leading causes of disability and it has no effective treatment. Spinal cord injury can also cause sensory impairment. With an impetus on using stem cells therapy in various central nervous system settings, there is an interest in using stem cells for addressing spinal cord injury. Neural stem cell is one type of stem cells that is able to differentiate to all three neural lineages and it shows promise in spinal injury treatment. Furthermore, a number of studies have shown that culturing NSCs in three-dimensional (3D) scaffolds like alginate could enhance neural differentiation.

methodsThe NSCs were isolated from 14-day-old rat embryos. The isolated NSCs were cultured in growth media containing basic fibroblast growth factor and endothelial growth factor. The cells were characterized by differentiating to three neural lineages and they were cultured in an alginate scaffold. After 7 days the cells were encapsulated and transplanted in a rat model of spinal cord injury.

resultsOur data showed that culturing in an alginate 3D scaffold and transplantation of the NSCs could improve neurological outcome in a rat model of spinal cord injury. The inflammation scores and lesion sizes and also the activity of caspase-3 (for apoptosis evaluation) were less in encapsulated neural stem cell transplantation cases.

conclusionsTransplantation of NSCs that were cultured in an alginate scaffold led to a better clinical and histological outcome for recovery from spinal cord injury in a rat model.

Indexed as

AlginateNeural stem cellsSpinal cord injuries

Identifiers

PMID27559438
PMCPMC4995241
OpenAlexW2510103591

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.