ArticleMolecular biology of the cell2016
Decoding the principles underlying the frequency of association with nucleoli for RNA polymerase III-transcribed genes in budding yeast.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 28 citations in OpenAlex.
- The selfish yeast plasmid exploits a SWI/SNF-type chromatin remodeling complex for hitchhiking on chromosomes and ensuring high-fidelity propagation.PLoS genetics · 2023Article
- Ex vivo visualization of RNA polymerase III-specific gene activity with electron microscopy.Communications biology · 2021Article
- Trans- and cis-acting effects of Firre on epigenetic features of the inactive X chromosome.Nature communications · 2020Article
- Quantitative Analysis of Spatial Distributions of All tRNA Genes in Budding Yeast.Biophysical journal · 2020Article
- Review
- Yeast PAF1 complex counters the pol III accumulation and replication stress on the tRNA genes.Scientific reports · 2019Article
- Impact of intron removal from tRNA genes on Saccharomyces cerevisiae.Nucleic acids research · 2019Article
- Condensin-Dependent Chromatin Compaction Represses Transcription Globally during Quiescence.Molecular cell · 2019Article
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- Retrotransposon targeting to RNA polymerase III-transcribed genes.Mobile DNA · 2018Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The association of RNA polymerase III (Pol III)-transcribed genes with nucleoli seems to be an evolutionarily conserved property of the spatial organization of eukaryotic genomes. However, recent studies of global chromosome architecture in budding yeast have challenged this view. We used live-cell imaging to determine the intranuclear positions of 13 Pol III-transcribed genes. The frequency of association with nucleolus and nuclear periphery depends on linear genomic distance from the tethering elements-centromeres or telomeres. Releasing the hold of the tethering elements by inactivating centromere attachment to the spindle pole body or changing the position of ribosomal DNA arrays resulted in the association of Pol III-transcribed genes with nucleoli. Conversely, ectopic insertion of a Pol III-transcribed gene in the vicinity of a centromere prevented its association with nucleolus. Pol III-dependent transcription was independent of the intranuclear position of the gene, but the nucleolar recruitment of Pol III-transcribed genes required active transcription. We conclude that the association of Pol III-transcribed genes with the nucleolus, when permitted by global chromosome architecture, provides nucleolar and/or nuclear peripheral anchoring points contributing locally to intranuclear chromosome organization.
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