ArticlePlant physiology2016
Glycosylphosphatidylinositol (GPI) Modification Serves as a Primary Plasmodesmal Sorting Signal.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- Differential transcriptomic landscapes of olive cultivars facing Xylella fastidiosa infection.BMC plant biology · 2026Article
- Should I stay or should I go-does protein localization to plasmodesmata depend on targeting signals?Journal of experimental botany · 2026Article
- LYM2 mediates chitin-induced plasmodesmal flux reduction inFrontiers in plant science · 2026Article
- Microscopic probing into plasmodesmata (PD) and PD-derived intercellular gateways (PdIGs): Beyond morphology.Protoplasma · 2026Review
- PDLP5, a plasmodesmata permeability regulator, can traffic between plant cells.NPJ science of plants · 2025Article
- New insights into plasmodesmata: complex 'protoplasmic connecting threads'.Journal of experimental botany · 2024Review
- JAGGER localization and function are dependent on GPI anchor addition.Plant reproduction · 2024Article
- Communicating Across Cell Walls: Structure, Evolution, and Regulation of Plasmodesmatal Transport in Plants.Results and problems in cell differentiation · 2024Review
- Heavy Metal-Associated Isoprenylated Plant Proteins (HIPPs) at Plasmodesmata: Exploring the Link between Localization and Function.Plants (Basel, Switzerland) · 2023Review
- Multi-copper oxidases SKU5 and SKS1 coordinate cell wall formation using apoplastic redox-based reactions in roots.Plant physiology · 2023Article
- Distinct functions of FASCILIN-LIKE ARABINOGALACTAN PROTEINS relate to domain structure.Plant physiology · 2023Article
- Individual lipid transfer proteins from Tanacetum parthenium show different specificity for extracellular accumulation of sesquiterpenes.Plant molecular biology · 2023Article
- Temporal transcriptome and metabolite analyses provide insights into the biochemical and physiological processes underlying endodormancy release in pistachio (Frontiers in plant science · 2023Article
- Promoter and domain structures regulate FLA12 function during Arabidopsis secondary wall development.Frontiers in plant science · 2023Article
- Glycosylphosphatidylinositol anchor lipid remodeling directs proteins to the plasma membrane and governs cell wall mechanics.The Plant cell · 2022Article
- Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Intercellular trafficking via plasmodesmata: molecular layers of complexity.Cellular and molecular life sciences : CMLS · 2021Review
- Targeted misexpression of NAC052, acting in H3K4 demethylation, alters leaf morphological and anatomical traits in Arabidopsis thaliana.Journal of experimental botany · 2020Article
- Organization and dynamics of functional plant membrane microdomains.Cellular and molecular life sciences : CMLS · 2020Review
- On the Potential Function of Type II ArabinogalactanFrontiers in plant science · 2020Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Plasmodesmata (Pd) are membranous channels that serve as a major conduit for cell-to-cell communication in plants. The Pd-associated β-1,3-glucanase (BG_pap) and CALLOSE BINDING PROTEIN1 (PDCB1) were identified as key regulators of Pd conductivity. Both are predicted glycosylphosphatidylinositol-anchored proteins (GPI-APs) carrying a conserved GPI modification signal. However, the subcellular targeting mechanism of these proteins is unknown, particularly in the context of other GPI-APs not associated with Pd Here, we conducted a comparative analysis of the subcellular targeting of the two Pd-resident and two unrelated non-Pd GPI-APs in Arabidopsis (Arabidopsis thaliana). We show that GPI modification is necessary and sufficient for delivering both BG_pap and PDCB1 to Pd Moreover, the GPI modification signal from both Pd- and non-Pd GPI-APs is able to target a reporter protein to Pd, likely to plasma membrane microdomains enriched at Pd As such, the GPI modification serves as a primary Pd sorting signal in plant cells. Interestingly, the ectodomain, a region that carries the functional domain in GPI-APs, in Pd-resident proteins further enhances Pd accumulation. However, in non-Pd GPI-APs, the ectodomain overrides the Pd targeting function of the GPI signal and determines a specific GPI-dependent non-Pd localization of these proteins at the plasma membrane and cell wall. Domain-swap analysis showed that the non-Pd localization is also dominant over the Pd-enhancing function mediated by a Pd ectodomain. In conclusion, our results indicate that segregation between Pd- and non-Pd GPI-APs occurs prior to Pd targeting, providing, to our knowledge, the first evidence of the mechanism of GPI-AP sorting in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.