Evidence map›Paper›PMID 27558782›Full record

ArticleJournal of industrial microbiology & biotechnology2016

Engineering levoglucosan metabolic pathway in Rhodococcus jostii RHA1 for lipid production.

Xiaochao Xiong, Jieni Lian, Xiaochen Yu, Manuel Garcia-Perez, Shulin Chen

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.1field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 35 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Article
  9. Review
  10. Metabolic Engineering of Oleaginous YeastFrontiers in microbiology · 2020
    Article
  11. Insights into the Metabolism of OleaginousApplied and environmental microbiology · 2019
    Review
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Xiaochao XiongDepartment of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA.
Jieni LianDepartment of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA.
Xiaochen YuDepartment of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA.
Manuel Garcia-PerezDepartment of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA.
Shulin ChenDepartment of Biological Systems Engineering, Washington State University, L. J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA. chens@wsu.edu.
Washington State University · USIowa State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oleaginous strains of Rhodococcus including R. jostii RHA1 have attracted considerable attention due to their ability to accumulate triacylglycerols (TAGs), robust growth properties and genetic tractability. In this study, a novel metabolic pathway was introduced into R. jostii by heterogenous expression of the well-characterized gene, lgk encoding levoglucosan kinase from Lipomyces starkeyi YZ-215. This enables the recombinant R. jostii RHA1 to produce TAGs from the anhydrous sugar, levoglucosan, which can be generated efficiently as the major molecule from the pyrolysis of cellulose. The recombinant R. jostii RHA1 could grow on levoglucosan as the sole carbon source, and the consumption rate of levoglucosan was determined. Furthermore, expression of one more copy of lgk increased the enzymatic activity of LGK in the recombinant. However, the growth performance of the recombinant bearing two copies of lgk on levoglucosan was not improved. Although expression of lgk in the recombinants was not repressed by the glucose present in the media, glucose in the sugar mixture still affected consumption of levoglucosan. Under nitrogen limiting conditions, lipid produced from levoglucosan by the recombinant bearing lgk was up to 43.54 % of the cell dry weight, which was comparable to the content of lipid accumulated from glucose. This work demonstrated the technical feasibility of producing lipid from levoglucosan, an anhydrosugar derived from the pyrolysis of lignocellulosic materials, by the genetically modified rhodococci strains.

Indexed as

CarbonGlucoseLipidsMetabolic EngineeringMetabolic Networks and PathwaysPhosphotransferasesRhodococcusTriglycerides1,6-anhydro-beta-glucopyranoseCarbonGlucoseLipidsPhosphotransferasesTriglyceridesLevoglucosanLignocellulosic biomassLipidPyrolysis oilRhodococcus jostii RHA1

Identifiers

PMID27558782
OpenAlexW2514340945

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.