ArticleScientific reports2016
Quantitative proteomic comparison of stationary/G0 phase cells and tetrads in budding yeast.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Stochastic phenotypic switching arises in response to directional selection in experimentally evolved multicellular yeast.Communications biology · 2025Article
- Chromosome duplication causes premature aging via defects in ribosome quality control.PLoS biology · 2025Article
- A Miniaturized Percoll Gradient Method for Isolation of Quiescent Cells of Yeast.Bio-protocol · 2025Article
- Premature aging in aneuploid yeast is caused in part by aneuploidy-induced defects in Ribosome Quality Control.bioRxiv : the preprint server for biology · 2024Article
- Riboproteome remodeling during quiescence exit iniScience · 2024Article
- Mitochondria complex I deficiency in Candida albicans arrests the cell cycle at S phase through suppressive TOR and PKA pathways.FEMS yeast research · 2024Article
- The yeast 14-3-3 proteins Bmh1 and Bmh2 regulate key signaling pathways.Frontiers in molecular biosciences · 2024Review
- Adaptive antimicrobial resistance, a description of microbial variants, and their relevance to periprosthetic joint infection.The bone & joint journal · 2022Review
- Cellular quiescence in budding yeast.Yeast (Chichester, England) · 2021Review
- Metabolic switches from quiescence to growth in synchronized Saccharomyces cerevisiae.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- 14-3-3 Proteins: a window for a deeper understanding of fungal metabolism and development.World journal of microbiology & biotechnology · 2019Review
- Differential abundance and transcription of 14-3-3 proteins during vegetative growth and sexual reproduction in budding yeast.Scientific reports · 2018Article
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2 authors.
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Abstract
Most of the microbial cells on earth under natural conditions exist in a dormant condition, commonly known as quiescent state. Quiescent cells exhibit low rates of transcription and translation suggesting that cellular abundance of proteins may be similar in quiescent cells. Therefore, this study aim to compare the proteome of budding yeast cells from two quiescent states viz. stationary phase/G0 and tetrads. Using iTRAQ (isobaric tag for relative and absolute quantitation) based quantitative proteomics we identified 289 proteins, among which around 40 proteins exhibited ±1.5 fold change consistently from the four biological replicates. Proteomics data was validated by western blot and denstiometric analysis of Hsp12 and Spg4. Level of budding yeast 14-3-3 proteins was found to be similar in both the quiescent states, whereas Hsp12 and Spg4 expressed only during stress. FACS (fluorescence-activated cell sorting) analysis showed that budding yeast cells were arrested at G1 stages both in tetrads as well as in stationary phase. We also observed that quiescent states did not express Ime1 (inducer of meiosis). Taken together, our present study demonstrates that the cells in quiescent state may have similar proteome, and accumulation of proteins like Hsp12, Hsp26, and Spg4 may play an important role in retaining viability of the cells during dormancy.
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