ArticleJournal of clinical microbiology2016
Accurate Detection of Avian Respiratory Viruses by Use of Multiplex PCR-Based Luminex Suspension Microarray Assay.
Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- A Multiplex Liquid Array Assay Based on Luminex xTAG Technology for the Detection of Five Respiratory Pathogens in SPF Chickens.Veterinary sciences · 2026Article
- Encoded Microspheres in Multiplex Detection of Mycotoxins and Other Analytes.Foods (Basel, Switzerland) · 2026Review
- Prevalence and Seroprevalence of Infectious Bronchitis Virus and Infectious Laryngotracheitis Virus in Backyard Poultry in Central Chile.Animals : an open access journal from MDPI · 2025Article
- Review of respiratory syndromes in poultry: pathogens, prevention, and control measures.Veterinary research · 2025Review
- Laboratory tools for the direct detection of bacterial respiratory infections and antimicrobial resistance: a scoping review.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Rapid detection of human adenovirus subgroup B using recombinase polymerase amplification assay.Virus genes · 2024Article
- Article
- Biotic concerns in generating molecular diagnosis matrixes for 4 avian viruses with emphasis on Marek's disease virus.Journal of virological methods · 2019Review
- Development of oligonucleotide microarray for accurate and simultaneous detection of avian respiratory viral diseases.BMC veterinary research · 2019Article
- High-throughput Luminex xMAP assay for simultaneous detection of antibodies against rabbit hemorrhagic disease virus, Sendai virus and rabbit rotavirus.Archives of virology · 2019Article
- Viral interference between low pathogenic avian influenza H9N2 and avian infectious bronchitis viruses in vitro and in ovo.Journal of virological methods · 2018Article
- Rapid diagnosis of human adenovirus B, C and E in the respiratory tract using multiplex quantitative polymerase chain reaction.Molecular medicine reports · 2018Article
- Diagnostic and Vaccination Approaches for Newcastle Disease Virus in Poultry: The Current and Emerging Perspectives.BioMed research international · 2018Review
- Multiplex Detection of Five Canine Viral Pathogens for Dogs as Laboratory Animals by the Luminex xTAG Assay.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel oligonucleotide suspension microarray (Luminex microsphere system) was developed for the rapid detection of avian respiratory viruses of major clinical importance. This test was optimized and validated with 70 clinical samples. The developed tool was accurate for high-throughput detection and differentiation of the most important avian respiratory viruses: avian influenza virus (AIV), Newcastle disease virus (NDV), infection bronchitis virus (IBV), and infectious laryngotracheitis virus (ILTV) in single- and mixed-virus infections. A multiplex reverse transcriptase PCR (RT-PCR), followed by a monoplex or a multiplex Luminex assays, were realized using a Luminex 200 analyzer instrument. The sensitivity, specificity, and reproducibility of the multiplex DNA suspension microarray system were evaluated. The results showed no significant differences in the median fluorescence intensity (MFI) value in monoplex and multiplex Luminex assays. The sensitivity and specificity proved to be completely concordant with monoplex real-time RT-PCR. We demonstrated that the multiplex DNA suspension microarray system is an accurate, high-throughput, and relatively simple method for the rapid detection of the main respiratory viruses of poultry.
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Registered trials
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