ArticleAnalytical and bioanalytical chemistry2016
Evaluation of the performance of quantitative detection of the Listeria monocytogenes prfA locus with droplet digital PCR.
Article in Analytical and bioanalytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Article
- Establishment of a dual droplet digital PCR method for detecting theFrontiers in microbiology · 2025Article
- Digital PCR threshold robustness analysis and optimization using dipcensR.Briefings in bioinformatics · 2024Article
- eDNA-based monitoring of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans with ddPCR in Luxembourg ponds: taking signals below the Limit of Detection (LOD) into account.BMC ecology and evolution · 2024Article
- Current methodologies available to evaluate the virulence potential amongFrontiers in microbiology · 2024Review
- Molecular Targets for Foodborne Pathogenic Bacteria Detection.Pathogens (Basel, Switzerland) · 2023Review
- Review
- Real-time polymerase chain reaction methods for strain specific identification and enumeration of strainFrontiers in microbiology · 2022Article
- Article
- Real-Time PCR Method Combined with a Matrix Lysis Procedure for the Quantification ofFoods (Basel, Switzerland) · 2021Article
- Austrian Raw-Milk Hard-Cheese Ripening Involves Successional Dynamics of Non-Inoculated Bacteria and Fungi.Foods (Basel, Switzerland) · 2020Article
- Comparison of the intensity of biofilm formation by Listeria monocytogenes using classical culture-based method and digital droplet PCR.AMB Express · 2020Article
- Rapid and Specific Detection ofFrontiers in microbiology · 2019Article
- Essential role of polymerases for assay performance - Impact of polymerase replacement in a well-established assay.Biomolecular detection and quantification · 2018Article
- Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- PCR-Stop analysis as a new tool for qPCR assay validation.Scientific reports · 2018Article
- Hydrophobic ionic liquids for quantitative bacterial cell lysis with subsequent DNA quantification.Analytical and bioanalytical chemistry · 2017Article
- Comparison among the Quantification of Bacterial Pathogens by qPCR, dPCR, and Cultural Methods.Frontiers in microbiology · 2017Article
- Article
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5 authors.
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Abstract
Fast and reliable pathogen detection is an important issue for human health. Since conventional microbiological methods are rather slow, there is growing interest in detection and quantification using molecular methods. The droplet digital polymerase chain reaction (ddPCR) is a relatively new PCR method for absolute and accurate quantification without external standards. Using the Listeria monocytogenes specific prfA assay, we focused on the questions of whether the assay was directly transferable to ddPCR and whether ddPCR was suitable for samples derived from heterogeneous matrices, such as foodstuffs that often included inhibitors and a non-target bacterial background flora. Although the prfA assay showed suboptimal cluster formation, use of ddPCR for quantification of L. monocytogenes from pure bacterial cultures, artificially contaminated cheese, and naturally contaminated foodstuff was satisfactory over a relatively broad dynamic range. Moreover, results demonstrated the outstanding detection limit of one copy. However, while poorer DNA quality, such as resulting from longer storage, can impair ddPCR, internal amplification control (IAC) of prfA by ddPCR, that is integrated in the genome of L. monocytogenes ΔprfA, showed even slightly better quantification over a broader dynamic range. Graphical Abstract Evaluating the absolute quantification potential of ddPCR targeting Listeria monocytogenes prfA.
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