ArticleParticle and fibre toxicology2016
Quantification of the cellular dose and characterization of nanoparticle transport during in vitro testing.
Article in Particle and fibre toxicology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed.
- In vitro dosimetry of multi-walled carbon nanotubes on human bronchial epithelial cells (BEAS-2B) using UV-Vis-NIR spectrophotometry and chemical digestion.Analytical and bioanalytical chemistry · 2026Article
- When conventional approach in toxicity assays falls short for nanomedicines: a case study with nanoemulsions.Drug delivery and translational research · 2025Article
- Nanotherapeutic Heterogeneity: Sources, Effects, and Solutions.Small (Weinheim an der Bergstrasse, Germany) · 2024Review
- Parenteral Lipid-Based Nanoparticles for CNS Disorders: Integrating Various Facets of Preclinical Evaluation towards More Effective Clinical Translation.Pharmaceutics · 2023Review
- Nanosafety: An Evolving Concept to Bring the Safest Possible Nanomaterials to Society and Environment.Nanomaterials (Basel, Switzerland) · 2022Review
- Guidance on risk assessment of nanomaterials to be applied in the food and feed chain: human and animal health.EFSA journal. European Food Safety Authority · 2021Article
- Understanding nano-engineered particle-cell interactions: biological insights from mathematical models.Nanoscale advances · 2021Review
- Quantification of Carbon Nanotube Doses in Adherent Cell Culture Assays Using UV-VIS-NIR Spectroscopy.Nanomaterials (Basel, Switzerland) · 2019Article
- Assessment of in vitro particle dosimetry models at the single cell and particle level by scanning electron microscopy.Journal of nanobiotechnology · 2018Article
- In vitro nanoparticle dosimetry for adherent growing cell monolayers covering bottom and lateral walls.Particle and fibre toxicology · 2018Article
- Guidance on risk assessment of the application of nanoscience and nanotechnologies in the food and feed chain: Part 1, human and animal health.EFSA journal. European Food Safety Authority · 2018Article
- The virtual cell based assay: Current status and future perspectives.Toxicology in vitro : an international journal published in association with BIBRA · 2017Article
- Erratum to: Quantification of the cellular dose and characterization of nanoparticle transport during in vitro testing.Particle and fibre toxicology · 2016Article
Corrections and comments
- Erratum issuedErratum to: Quantification of the cellular dose and characterization of nanoparticle transport during in vitro testing.2016 · Urbán, Patrizia [corrected to Urbán, Patricia]
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe constant increase of the use of nanomaterials in consumer products is making increasingly urgent that standardized and reliable in vitro test methods for toxicity screening be made available to the scientific community. For this purpose, the determination of the cellular dose, i.e. the amount of nanomaterials effectively in contact with the cells is fundamental for a trustworthy determination of nanomaterial dose responses. This has often been overlooked in the literature making it difficult to undertake a comparison of datasets from different studies. Characterization of the mechanisms involved in nanomaterial transport and the determination of the cellular dose is essential for the development of predictive numerical models and reliable in vitro screening methods.
resultsThis work aims to relate key physico-chemical properties of gold nanoparticles (NPs) to the kinetics of their deposition on the cellular monolayer. Firstly, an extensive characterization of NPs in complete culture cell medium was performed to determine the diameter and the apparent mass density of the formed NP-serum protein complexes. Subsequently, the kinetics of deposition were studied by UV-vis absorbance measurements in the presence or absence of cells. The fraction of NPs deposited on the cellular layer was found to be highly dependent on NP size and apparent density because these two parameters influence the NP transport. The NP deposition occurred in two phases: phase 1, which consists of cellular uptake driven by the NP-cell affinity, and phase 2 consisting mainly of NP deposition onto the cellular membrane.
conclusionThe fraction of deposited NPs is very different from the initial concentration applied in the in vitro assay, and is highly dependent of the size and density of the NPs, on the associated transport rate and on the exposure duration. This study shows that an accurate characterization is needed and suitable experimental conditions such as initial concentration of NPs and liquid height in the wells has to be considered since they strongly influence the cellular dose and the nature of interactions of NPs with the cells.
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