ArticleJournal of ovarian research2016
Long-term storage does not impact the quality of cryopreserved human ovarian tissue.
Article in Journal of ovarian research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Transport Time Does Not Substantially Alter RNA Expression in Human Ovarian Tissue After Standardized Slow-Freezing for Fertility Preservation.Journal of clinical medicine · 2026Article
- Hydrogen-generating nanomodulators for metabolic repair and functional recovery in ovarian tissue transplantation.Journal of nanobiotechnology · 2026Article
- Cryopreservation of ovarian tissue - what's known so far and future perspectives.Frontiers in reproductive health · 2026Review
- Ovarian tissue transplantation: 10 years of experience at the Bologna University.Frontiers in endocrinology · 2024Observational
- Ovarian tissue cryopreservation and transplantation: 20 years experience in Bologna University.Frontiers in endocrinology · 2022Article
- Tissue fixation effects on human retinal lipid analysis by MALDI imaging and LC-MS/MS technologies.Journal of mass spectrometry : JMS · 2021Article
- Improved preservation of ovarian tissue morphology that is compatible with antigen detection using a fixative mixture of formalin and acetic acid.Human reproduction (Oxford, England) · 2021Article
- Effect of resveratrol on mouse ovarian vitrification and transplantation.Reproductive biology and endocrinology : RB&E · 2021Article
- Electrospun patterned porous scaffolds for the support of ovarian follicles growth: a feasibility study.Scientific reports · 2019Article
- Retrievable hydrogels for ovarian follicle transplantation and oocyte collection.Biotechnology and bioengineering · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOvarian tissue cryopreservation is an emerging technique, also addressed to very young cancer patients, for whom it is not possible to perform an ovarian stimulation for oocytes freezing, before gonadotoxic treatment. In this cases, ovarian tissue must be cryopreserved for a long period of time and it is very important to know if it maintains fertility function after a long period of storage. Here we aimed to assess the effect of long-term storage on preservation and viability of cryopreserved human ovarian tissue.
methodsDescriptive study of three cases of cancer patients whose cryopreserved ovarian tissue remained stored for 18 years. Long-term stored tissue was examined by histological and immunohistochemical analysis, transmission electron microscopy, TUNEL assay and LIVE/DEAD viability/citotoxicity test.
resultsOvarian tissue stored for 18 years showed a good morphology. Follicles presented negative staining for estrogen and progesterone receptors, positive staining for ki67 in granulosa cells and/or oocytes and for bcl2 in granulosa cells. Regarding stroma, patch/focal positive expression was found for estrogen receptor and ki67, diffusely positive expression for progesterone receptor and bcl2. After long-term storage, ultrastructural examination showed sub-cellular integrity of follicles and interstitial oedema foci. No apoptosis was observable by TUNEL assay. Stromal cell viability remained >97 % during the culture period.
conclusionThe evaluation of different aspects of the tissue provides evidence that the storage time does not impact on tissue quality and gives hope especially to cancer girls, whose tissues could remain cryopreserved for a very long time.
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