ArticleMicrobial cell factories2016
Engineering Corynebacterium glutamicum for violacein hyper production.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 70 citations in OpenAlex.
- Engineering yeast for sustainable bioproduction of tryptophan-derived aromatic compounds.FEMS yeast research · 2026Review
- Bioconversion of a Dairy By-Product (Microorganisms · 2025Article
- A comprehensive review on violacein production by microbial fermentation.Biodesign research · 2025Review
- Production, downstream, stability, bioactivities, and applications studies of violacein and related compounds.World journal of microbiology & biotechnology · 2025Review
- Production of aromatic amino acids and their derivatives by Escherichia coli and Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Review
- Stability of a MutualisticEngineering in life sciences · 2024Article
- Review of the Proteomics and Metabolic Properties ofMicroorganisms · 2024Review
- Pigments from pathogenic bacteria: a comprehensive update on recent advances.World journal of microbiology & biotechnology · 2024Review
- Expanding the application of tryptophan: Industrial biomanufacturing of tryptophan derivatives.Frontiers in microbiology · 2023Review
- Improvement in Violacein Production by Utilizing Formic Acid to Induce Quorum Sensing inAntioxidants (Basel, Switzerland) · 2022Article
- Cell-Free PURE System: Evolution and Achievements.Biodesign research · 2022Review
- Developing methylotrophic microbial platforms for a methanol-based bioindustry.Frontiers in bioengineering and biotechnology · 2022Review
- Recent Advances in Synthetic, Industrial and Biological Applications of Violacein and Its Heterologous Production.Journal of microbiology and biotechnology · 2021Review
- Biotechnological Activities and Applications of Bacterial Pigments Violacein and Prodigiosin.Journal of biological engineering · 2021Review
- Direct RBS Engineering of the biosynthetic gene cluster for efficient productivity of violaceins in E. coli.Microbial cell factories · 2021Article
- Development and comparison of cell-free protein synthesis systems derived from typical bacterial chassis.Bioresources and bioprocessing · 2021Article
- EngineeringACS synthetic biology · 2020Article
- Recent Advances in Metabolically Engineered Microorganisms for the Production of Aromatic Chemicals Derived From Aromatic Amino Acids.Frontiers in bioengineering and biotechnology · 2020Review
- Article
- Metabolic engineering of microorganisms for production of aromatic compounds.Microbial cell factories · 2019Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCorynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to Corynebacterium's GRAS status and advantages in tryptophan fermentation. The violacein is a commercially potential compound with various applications derived from L-tryptophan.
resultsCorynebacterium glutamicum ATCC 21850 that could produce 162.98 mg L(-1) tryptophan was employed as a novel host for metabolic engineering chassis. Heterologous vio operon from Chromobacterium violaceum was over-expressed in ATCC 21850 strain with constitutive promoter to have obtained 532 mg L(-1) violacein. Considering toxicity of violacein, vio operon was expressed with inducible promoter and 629 mg L(-1) violacein was obtained in batch culture. Due to the economical coding nature of vio operon, the compressed RBS of vio genes were replaced with complete strong C. glutamicum ones. And extended expression units were assembled to form a synthetic operon. With this strategy, 1116 mg L(-1) violacein in batch culture was achieved. Fermentation process was then optimized by studying induction time, induction concentration, culture composition and fermentation temperature. as a result, a titer of 5436 mg L(-1) and a productivity of 47 mg L(-1) h(-1) were achieved in 3 L bioreactor.
conclusionsWith metabolic engineering and fermentation optimization practice, C. glutamicum 21850 (pEC-C-vio1) was able to produce violacein with both titer and productivity at the highest level ever reported. Due to advantages of mature C. glutamicum fermentation industry, this work has built basis for commercial production of violacein.
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