ArticlePloS one2016
Revisiting Mitochondrial pH with an Improved Algorithm for Calibration of the Ratiometric 5(6)-carboxy-SNARF-1 Probe Reveals Anticooperative Reaction with H+ Ions and Warrants Further Studies of Organellar pH.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 24 citations in OpenAlex.
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- Advances in Small-Molecule Fluorescent pH Probes for Monitoring Mitophagy.Chemical & biomedical imaging · 2024Review
- Super-Resolution Imaging of Voltages in the Interior of Individual, Vital Mitochondria.ACS nano · 2024Article
- Driving electrochemical reactions at the microscale using CMOS microelectrode arrays.Lab on a chip · 2023Article
- Network representation and analysis of energy coupling mechanisms in cellular metabolism by a graph-theoretical approach.Theory in biosciences = Theorie in den Biowissenschaften · 2022Article
- Ni(II) Ions May Target the Entire Melatonin Biosynthesis Pathway-A Plausible Mechanism of Nickel Toxicity.Molecules (Basel, Switzerland) · 2022Article
- Mitochondrial energetics with transmembrane electrostatically localized protons: do we have a thermotrophic feature?Scientific reports · 2021Article
- Energy Renewal: Isothermal Utilization of Environmental Heat Energy with Asymmetric Structures.Entropy (Basel, Switzerland) · 2021Article
- The Proton in Biochemistry: Impacts on Bioenergetics, Biophysical Chemistry, and Bioorganic Chemistry.Frontiers in molecular biosciences · 2021Review
- Development of photo- and chemo-stable near-infrared-emitting dyes: linear-shape benzo-rosol and its derivatives as unique ratiometric bioimaging platforms.Chemical science · 2020Article
- An update of the chemiosmotic theory as suggested by possible proton currents inside the coupling membrane.Open biology · 2019Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence measurements of pH and other analytes in the cell rely on accurate calibrations, but these have routinely used algorithms that inadequately describe the properties of indicators. Here, we have established a more accurate method for calibrating and analyzing data obtained using the ratiometric probe 5(6)-carboxy-SNARF-1. We tested the implications of novel approach to measurements of pH in yeast mitochondria, a compartment containing a small number of free H+ ions. Our findings demonstrate that 5(6)-carboxy-SNARF-1 interacts with H+ ions inside the mitochondria in an anticooperative manner (Hill coefficient n of 0.5) and the apparent pH inside the mitochondria is ~0.5 unit lower than had been generally assumed. This result, at odds with the current consensus on the mechanism of energy generation in the mitochondria, is in better agreement with theoretical considerations and warrants further studies of organellar pH.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.