Evidence map›Paper›PMID 27557119›Full record

ArticleBMC genomics2016

Whole genomic sequence analysis of Bacillus infantis: defining the genetic blueprint of strain NRRL B-14911, an emerging cardiopathogenic microbe.

Chandirasegaran Massilamany, Akram Mohammed, John Dustin Loy, Tanya Purvis, Bharathi Krishnan, Rakesh H Basavalingappa, Christy M Kelley, Chittibabu Guda, Raúl G Barletta, Etsuko N Moriyama and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

Chandirasegaran MassilamanySchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Akram MohammedUniversity of Nebraska Medical Center, Omaha, NE, 68198, USA.
John Dustin LoySchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Tanya PurvisKansas State Veterinary Diagnostic Laboratory, Manhattan, KS, 66506, USA.
Bharathi KrishnanSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Rakesh H BasavalingappaSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Christy M KelleyGenetics, Breeding and Animal Health Unit, U.S. Meat Animal Research Center, Clay Center, NE, 68933, USA.
Chittibabu GudaUniversity of Nebraska Medical Center, Omaha, NE, 68198, USA.
Raúl G BarlettaSchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Etsuko N MoriyamaSchool of Biological Sciences and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
Timothy P L SmithGenetics, Breeding and Animal Health Unit, U.S. Meat Animal Research Center, Clay Center, NE, 68933, USA.
Jay ReddySchool of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA. nreddy2@unl.edu.
University of Nebraska–Lincoln · USRoman L. Hruska U.S. Meat Animal Research Center · USUniversity of Nebraska Medical Center · USKansas State University · US

Funding

Nebraska Center for VirologyP30GM103509 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI WOOD, CHARLES · 2012 to 2014
$3.3M
Autoimmunity in the Mediation of Infectious MyocarditisR01HL114669 · NHLBI · UNIVERSITY OF NEBRASKA LINCOLN · PI REDDY, JAY · 2013 to 2016
$1.4M
NHLBI NIH HHS R01 HL114669NIGMS NIH HHS P30 GM103509
6 · The paper itself

Abstract

backgroundWe recently reported the identification of Bacillus sp. NRRL B-14911 that induces heart autoimmunity by generating cardiac-reactive T cells through molecular mimicry. This marine bacterium was originally isolated from the Gulf of Mexico, but no associations with human diseases were reported. Therefore, to characterize its biological and medical significance, we sought to determine and analyze the complete genome sequence of Bacillus sp. NRRL B-14911.

resultsBased on the phylogenetic analysis of 16S ribosomal RNA (rRNA) genes, sequence analysis of the 16S-23S rDNA intergenic transcribed spacers, phenotypic microarray, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry, we propose that this organism belongs to the species Bacillus infantis, previously shown to be associated with sepsis in a newborn child. Analysis of the complete genome of Bacillus sp. NRRL B-14911 revealed several virulence factors including adhesins, invasins, colonization factors, siderophores and transporters. Likewise, the bacterial genome encodes a wide range of methyl transferases, transporters, enzymatic and biochemical pathways, and insertion sequence elements that are distinct from other closely related bacilli.

conclusionsThe complete genome sequence of Bacillus sp. NRRL B-14911 provided in this study may facilitate genetic manipulations to assess gene functions associated with bacterial survival and virulence. Additionally, this bacterium may serve as a useful tool to establish a disease model that permits systematic analysis of autoimmune events in various susceptible rodent strains.

Indexed as

BacillusGenome, BacterialGenomicsHeartHumansMolecular Sequence AnnotationMyocardiumPhenotypePhylogenyRNA, Ribosomal, 16SSequence Analysis, DNARNA, Ribosomal, 16SBacillus sp. NRRL B-14911GenomeHeart

Identifiers

PMID27557119
PMCPMC5001198
OpenAlexW2514064007

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.