ArticlePloS one2016
Cold Shock Proteins Are Expressed in the Retina Following Exposure to Low Temperatures.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Therapeutic Levels of Hypothermia Achieved in Isolated Porcine Eyes Using a Scleral Contact Interface.Annals of biomedical engineering · 2026Article
- Cold-shock proteins accumulate in centrosomes and their expression and primary cilium morphology are regulated by hypothermia and shear stress.Histology and histopathology · 2024Article
- A hypothermia mimetic molecule (zr17-2) reduces ganglion cell death, gliosis, and electroretinogram distortion in male rats subjected to perinatal asphyxia.Frontiers in pharmacology · 2023Article
- Selective intraarterial hypothermia combined with mechanical thrombectomy for acute cerebral infarction based on microcatheter technology: A single-center, randomized, single-blind controlled study.Frontiers in neurology · 2023Article
- A hypothermia mimetic molecule (zr17-2) reduces ganglion cell death and electroretinogram distortion in a rat model of intraorbital optic nerve crush (IONC).Frontiers in pharmacology · 2023Article
- Mild Therapeutic Hypothermia and Putative Mechanisms of Hair Cell Survival in the Cochlea.Antioxidants & redox signaling · 2022Article
- Cold protection allows local cryotherapy in a clinical-relevant model of traumatic optic neuropathy.eLife · 2022Article
- Associations between RNA-Binding Motif Protein 3, Fibroblast Growth Factor 21, and Clinical Outcome in Patients with Stroke.Journal of clinical medicine · 2022Article
- Hypothermic Shock Applied After Perinatal Asphyxia Prevents Retinal Damage in Rats.Frontiers in pharmacology · 2021Article
- Research Progress of the Application of Hypothermia in the Eye.Oxidative medicine and cellular longevity · 2020Review
- Review
- Extracellular CIRP (eCIRP) and inflammation.Journal of leukocyte biology · 2019Review
- Plasma Cold-Inducible RNA-Binding Protein Predicts Lung Dysfunction After Cardiovascular Surgery Following Cardiopulmonary Bypass: A Prospective Observational Study.Medical science monitor : international medical journal of experimental and clinical research · 2019Observational
- A New Vision for Therapeutic Hypothermia in the Era of Targeted Temperature Management: A Speculative Synthesis.Therapeutic hypothermia and temperature management · 2019Review
- A Stress Response that Monitors and Regulates mRNA Structure Is Central to Cold Shock Adaptation.Molecular cell · 2018Article
- Infants Uniquely Express High Levels of RBM3 and Other Cold-Adaptive Neuroprotectant Proteins in the Human Brain.Developmental neuroscience · 2018Article
- Recent progress in the research of cold-inducible RNA-binding protein.Future science OA · 2017Review
- Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hypothermia has been proposed as a therapeutic intervention for some retinal conditions, including ischemic insults. Cold exposure elevates expression of cold-shock proteins (CSP), including RNA-binding motif protein 3 (RBM3) and cold inducible RNA-binding protein (CIRP), but their presence in mammalian retina is so far unknown. Here we show the effects of hypothermia on the expression of these CSPs in retina-derived cell lines and in the retina of newborn and adult rats. Two cell lines of retinal origin, R28 and mRPE, were exposed to 32°C for different time periods and CSP expression was measured by qRT-PCR and Western blotting. Neonatal and adult Sprague-Dawley rats were exposed to a cold environment (8°C) and expression of CSPs in their retinas was studied by Western blotting, multiple inmunofluorescence, and confocal microscopy. RBM3 expression was upregulated by cold in both R28 and mRPE cells in a time-dependent fashion. On the other hand, CIRP was upregulated in R28 cells but not in mRPE. In vivo, expression of CSPs was negligible in the retina of newborn and adult rats kept at room temperature (24°C). Exposure to a cold environment elicited a strong expression of both proteins, especially in retinal pigment epithelium cells, photoreceptors, bipolar, amacrine and horizontal cells, Müller cells, and ganglion cells. In conclusion, CSP expression rapidly rises in the mammalian retina following exposure to hypothermia in a cell type-specific pattern. This observation may be at the basis of the molecular mechanism by which hypothermia exerts its therapeutic effects in the retina.
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