ArticleBiotechnology for biofuels2016
Elucidation of the co-metabolism of glycerol and glucose in Escherichia coli by genetic engineering, transcription profiling, and (13)C metabolic flux analysis.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 35 citations in OpenAlex.
- Antibacterial mechanisms of phenyllactic acid againstFrontiers in nutrition · 2026Article
- Induction conditions that promote the effect of glycerol on recombinant protein production inBiotechnology reports (Amsterdam, Netherlands) · 2025Article
- Increasing the Pentose Phosphate Pathway Flux to Improve Plasmid DNA Production in EngineeredMicroorganisms · 2024Article
- Community-specific diffusion characteristics determine resistance of biofilms to oxidative stress.Science advances · 2023Article
- Growth productivity as a determinant of the inoculum effect for bactericidal antibiotics.Science advances · 2022Article
- Cascaded processing enables continuous upstream processing with E. coli BL21(DE3).Scientific reports · 2021Article
- Designing next generation recombinant protein expression platforms by modulating the cellular stress response in Escherichia coli.Microbial cell factories · 2020Article
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- The LazarusFrontiers in bioengineering and biotechnology · 2020Article
- Quantifying Acute Fuel and Respiration Dependent pH Homeostasis in Live Cells Using the mCherryTYG Mutant as a Fluorescence Lifetime Sensor.Analytical chemistry · 2019Article
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- Rewiring glycerol metabolism for enhanced production of poly-γ-glutamic acid inBiotechnology for biofuels · 2018Article
- Engineering NOG-pathway in Escherichia coli for poly-(3-hydroxybutyrate) production from low cost carbon sources.Bioengineered · 2018Article
- ExaminingBiotechnology for biofuels · 2016Article
- Editorial: chemicals and bioproducts from biomass.Biotechnology for biofuels · 2016Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGlycerol, a byproduct of biodiesel, has become a readily available and inexpensive carbon source for the production of high-value products. However, the main drawback of glycerol utilization is the low consumption rate and shortage of NADPH formation, which may limit the production of NADPH-requiring products. To overcome these problems, we constructed a carbon catabolite repression-negative ΔptsGglpK* mutant by both blocking a key glucose PTS transporter and enhancing the glycerol conversion. The mutant can recover normal growth by co-utilization of glycerol and glucose after loss of glucose PTS transporter. To reveal the metabolic potential of the ΔptsGglpK* mutant, this study examined the flux distributions and regulation of the co-metabolism of glycerol and glucose in the mutant.
resultsBy labeling experiments using [1,3-(13)C]glycerol and [1-(13)C]glucose, (13)C metabolic flux analysis was employed to decipher the metabolisms of both the wild-type strain and the ΔptsGglpK* mutant in chemostat cultures. When cells were maintained at a low dilution rate (0.1 h(-1)), the two strains showed similar fluxome profiles. When the dilution rate was increased, both strains upgraded their pentose phosphate pathway, glycolysis and anaplerotic reactions, while the ΔptsGglpK* mutant was able to catabolize much more glycerol than glucose (more than tenfold higher). Compared with the wild-type strain, the mutant repressed its flux through the TCA cycle, resulting in higher acetate overflow. The regulation of fluxomes was consistent with transcriptional profiling of several key genes relevant to the TCA cycle and transhydrogenase, namely gltA, icdA, sdhA and pntA. In addition, cofactor fluxes and their pool sizes were determined. The ΔptsGglpK* mutant affected the redox NADPH/NADH state and reduced the ATP level. Redox signaling activated the ArcA regulatory system, which was responsible for TCA cycle repression.
conclusionsThis work employs both (13)C-MFA and transcription/metabolite analysis for quantitative investigation of the co-metabolism of glycerol and glucose in the ΔptsGglpK* mutant. The ArcA regulatory system dominates the control of flux redistribution. The ΔptsGglpK* mutant can be used as a platform for microbial cell factories for the production of biofuels and biochemicals, since most of fuel molecule (e.g., alcohols) synthesis requires excess reducing equivalents.
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