ArticleScientific reports2016
A mutagenic study identifying critical residues for the structure and function of rice manganese transporter OsMTP8.1.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- A Genome‑wide Association Study of the Grain Ionome in Rice Oryza Sativa Ssp. Japonica under Two Diverse Water Management Systems.Rice (New York, N.Y.) · 2025Article
- Engineering rice Nramp5 modifies cadmium and manganese uptake selectivity using yeast assay system.Frontiers in plant science · 2024Article
- Dissecting the relative contribution of ECA3 and group 8/9 cation diffusion facilitators to manganese homeostasis inPlant direct · 2023Article
- Article
- Genome-Wide Identification, Structure Characterization, Expression Pattern Profiling, and Substrate Specificity of the Metal Tolerance Protein Family inPlants (Basel, Switzerland) · 2021Article
- Genome-wide association mapping for grain manganese in rice (Oryza sativa L.) using a multi-experiment approach.Heredity · 2021Article
- Characterization of Metal Tolerance Proteins and Functional Analysis of GmMTP8.1 Involved in Manganese Tolerance in Soybean.Frontiers in plant science · 2021Article
- Genome-Wide Identification of Metal Tolerance Protein Genes inInternational journal of molecular sciences · 2020Article
- Disruption of the OLE ribonucleoprotein complex causes magnesium toxicity in Bacillus halodurans.Molecular microbiology · 2019Article
- Comprehensive Analysis of the Cadmium Tolerance of Abscisic Acid-, Stress- and Ripening-Induced Proteins (ASRs) in Maize.International journal of molecular sciences · 2019Article
- Comparative analysis of Cd-responsive maize and rice transcriptomes highlights Cd co-modulated orthologs.BMC genomics · 2018Article
- OsMTP11 is localised at the Golgi and contributes to Mn tolerance.Scientific reports · 2017Article
- The Arabidopsis MTP8 transporter determines the localization of manganese and iron in seeds.Scientific reports · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rice (Oryza sativa) MTP8.1 (OsMTP8.1) is a tonoplast-localized manganese transporter of the cation diffusion facilitator family. Here we present a structure-function analysis of OsMTP8.1 based on the site-directed and random mutagenesis and complementation assays in manganese hypersensitive yeast, in combination with three-dimensional (3D) structure modeling based on the crystal structure of the Escherichia coli CDF family member, EcYiiP. Two metal-binding sites are conserved in OsMTP8.1 with EcYiiP, one is between transmembrane helices TM2 and TM5, the other is the cytoplasmic C-terminus. In addition to these two metal-binding sites, there may exist other Mn-binding sites such as that at the very end of the CTD. Two residues (R167 and L296) may play an important role for the hinge-like movement of CTDs. Several mutations such as E357A and V374D may affect dimer formation, and S132A may induce a conformational change, resulting in a loss of transport function or modification in metal selectivity. The N-terminus of OsMTP8.1 was not functional for Mn transport activity, and the real function of NTD remains to be investigated in the future. The findings of the present study illustrate the structure-function relationship of OsMTP8.1 in Mn transport activity, which may also be applied to other plant Mn-CDF proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.