ArticleThe Journal of biological chemistry2016
Structural and Functional Basis for Lipid Synergy on the Activity of the Antibacterial Peptide ABC Transporter McjD.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Chemoenzymatic Modification of Microcin J25 with Single-Residue Precision Provides New-to-Nature Lasso Peptides.JACS Au · 2026Article
- Precursor gene engineering expands the loop region of the lasso peptide microcin J25.RSC chemical biology · 2026Article
- Decoupling growth phase dependency and metal ion inhibition: A dual engineering strategy for the high-yield biosynthesis of microcin J25 inEngineering microbiology · 2025Article
- Structure of the human respiratory complex II.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A molecular switch controls the impact of cholesterol on a Kir channel.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Model architectures for bacterial membranes.Biophysical reviews · 2022Review
- Molecular mechanism of SbmA, a promiscuous transporter exploited by antimicrobial peptides.Science advances · 2021Article
- Assessing the Role of Lipids in the Molecular Mechanism of Membrane Proteins.International journal of molecular sciences · 2021Review
- Sequential purification and characterization of Torpedo californica nAChR-DC supplemented with CHS for high-resolution crystallization studies.Analytical biochemistry · 2020Article
- Characterization of Lipid-Protein Interactions and Lipid-Mediated Modulation of Membrane Protein Function through Molecular Simulation.Chemical reviews · 2019Review
- Emerging Diversity in Lipid-Protein Interactions.Chemical reviews · 2019Review
- Computational Modeling of Realistic Cell Membranes.Chemical reviews · 2019Review
- The Different Effects of Substrates and Nucleotides on the Complex Formation of ABC Transporters.Structure (London, England : 1993) · 2019Article
- Solid immersion microscopy images cells under cryogenic conditions with 12 nm resolution.Communications biology · 2019Article
- Conformational dynamics of the ABC transporter McjD seen by single-molecule FRET.The EMBO journal · 2018Article
- N-glycan microheterogeneity regulates interactions of plasma proteins.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Structural Lipids Enable the Formation of Functional Oligomers of the Eukaryotic Purine Symporter UapA.Cell chemical biology · 2018Article
- Coarse-grained molecular dynamics simulations reveal lipid access pathways in P-glycoprotein.The Journal of general physiology · 2018Article
- Structural basis for antibacterial peptide self-immunity by the bacterial ABC transporter McjD.The EMBO journal · 2017Article
- Structure of the transporter associated with antigen processing trapped by herpes simplex virus.eLife · 2016Article
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11 authors.
Funding
Abstract
The lipid bilayer is a dynamic environment that consists of a mixture of lipids with different properties that regulate the function of membrane proteins; these lipids are either annular, masking the protein hydrophobic surface, or specific lipids, essential for protein function. In this study, using tandem mass spectrometry, we have identified specific lipids associated with the Escherichia coli ABC transporter McjD, which translocates the antibacterial peptide MccJ25. Using non-denaturing mass spectrometry, we show that McjD in complex with MccJ25 survives the gas phase. Partial delipidation of McjD resulted in reduced ATPase activity and thermostability as shown by circular dichroism, both of which could be restored upon addition of defined E. coli lipids. We have resolved a phosphatidylglycerol lipid associated with McjD at 3.4 Å resolution, whereas molecular dynamic simulations carried out in different lipid environments assessed the binding of specific lipids to McjD. Combined, our data show a synergistic effect of zwitterionic and negatively charged lipids on the activity of McjD; the zwitterionic lipids provide structural stability to McjD, whereas the negatively charged lipids are essential for its function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.