ArticleKidney international2016
Label-free fluorescence lifetime and second harmonic generation imaging microscopy improves quantification of experimental renal fibrosis.
Article in Kidney international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
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44 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Nonlinear Microscopy of ECM Remodeling in Renal and Vascular Tissues: A Systematic Review Integrating Human AVF Imaging.Medicina (Kaunas, Lithuania) · 2026Pooled it
- Non-invasive assessment techniques for renal fibrosis: advances and perspectives.Renal failure · 2025Review
- Nonlinear Imaging Detection of Organ Fibrosis in Minute Samples for Early Stage Utilizing Dual-Channel Two-Photon and Second-Harmonic Excitation.Biosensors · 2025Article
- Acute kidney injury results in long-term alterations of kidney lymphatics in mice.American journal of physiology. Renal physiology · 2024Article
- Nascent shifts in renal cellular metabolism, structure, and function due to chronic empagliflozin in prediabetic mice.American journal of physiology. Cell physiology · 2024Article
- Advances in imaging techniques to assess kidney fibrosis.Renal failure · 2023Review
- Fibrosis quantification using multiphoton excitation imaging of picrosirius red stained cardiac tissue.Research square · 2023Article
- The TNFThe American journal of pathology · 2023Article
- Label-free fluorescence microscopy: revisiting the opportunities with autofluorescent molecules and harmonic generations as biosensors and biomarkers for quantitative biology.Biophysical reviews · 2023Review
- A roadmap for developing and engineeringBiophysics reviews · 2023Review
- Article
- Sphingosine kinase 1 mediates sexual dimorphism in fibrosis in a mouse model of NASH.Molecular metabolism · 2022Article
- Empagliflozin Treatment Attenuates Hepatic Steatosis by Promoting White Adipose Expansion in Obese TallyHo Mice.International journal of molecular sciences · 2022Article
- Label-free imaging of non-deparaffinized sections of the human kidney to determine tissue quality and signatures of disease.Physiological reports · 2022Article
- Intravital Multiphoton Microscopy as a Tool for Studying Renal Physiology, Pathophysiology and Therapeutics.Frontiers in physiology · 2022Review
- Label-free, fast, 2-photon volume imaging of the organization of neurons and glia in the enteric nervous system.Frontiers in neuroanatomy · 2022Article
- Oral N-acetylcysteine decreases IFN-γ production and ameliorates ischemia-reperfusion injury in steatotic livers.Frontiers in immunology · 2022Article
- 15-Lipoxygenase worsens renal fibrosis, inflammation, and metabolism in a murine model of ureteral obstruction.American journal of physiology. Renal physiology · 2022Article
- Genes, other than Muc5b, play a role in bleomycin-induced lung fibrosis.American journal of physiology. Lung cellular and molecular physiology · 2021Article
- Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline.Laboratory investigation; a journal of technical methods and pathology · 2021Article
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Abstract
All forms of progressive renal diseases develop a final pathway of tubulointerstitial fibrosis and glomerulosclerosis. Renal fibrosis is usually quantified using histological staining, a process that is time-consuming and pathologist dependent. Here we develop a fast and operator-independent method to measure fibrosis utilizing the murine unilateral ureteral obstruction model which manifests a time-dependent fibrotic increase in obstructed kidneys while the contralateral kidneys are used as controls. After ureteral obstruction, kidneys were analyzed at 7, 14, and 21 days. Fibrosis was quantified using fluorescence lifetime imaging (FLIM) and second harmonic generation (SHG) in a Deep Imaging via Enhanced photon Recovery deep tissue imaging microscope. This microscope was developed for deep tissue along with second and third harmonic generation imaging and has extraordinary sensitivity toward harmonic generation. SHG data suggest the presence of more fibrillar collagen in the obstructed kidneys. The combination of short-wavelength FLIM and SHG analysis results in a robust assessment procedure independent of observer interpretation and let us create criteria to quantify the extent of fibrosis directly from the image. Thus, the FLIM-SHG technique shows remarkable improvement in quantification of renal fibrosis compared to standard histological techniques.
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