Evidence map›Paper›PMID 27554369›Full record

ArticleJournal of molecular histology2016

A zebrafish mosaic assay to study mammalian stem cells in real time in vivo.

Chun Xiao, Meilin Qian, Chaoran Yin, Yonggang Zhang, Huozhen Hu, Shaohua Yao

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular histology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Adult neural stem cell plasticity.Neural regeneration research · 2019
    Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Chun XiaoCollege of life Science and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Meilin QianCollege of life Science and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Chaoran YinCollege of life Science and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Yonggang ZhangCollege of life Science and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Huozhen HuCollege of life Science and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China. huhuozhen_sc@163.com.
Shaohua YaoCollege of life Science and State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The differentiation potentials of stem cells have been evaluated by various in vivo and in vitro assays. However, these assays have different limitations hindering efficient study of mammalian stem cells. Here we describe a rapid and powerful mosaic assay to study the differentiation potentials of stem cells in real time in vivo by using zebrafish embryo. We transplanted mouse neural stem cells into zebrafish embryos at different developmental stages and found that they mainly formed neural tissues while occasionally trans-differentiated into mesoderm- and endoderm-derived tissues. Because zebrafish embryo is transparent, the behaviors of transplanted mouse stem cells can be easily tracked in a real-time manner and at single-cell resolution. We expect that this assay may be widely applied to explore the in vivo behaviors of any stem cells available.

Indexed as

MosaicismAnimalsAnimals, Genetically ModifiedCell Culture TechniquesCell DifferentiationCell TransdifferentiationEndodermMesodermMiceNeural Stem CellsStem CellsZebrafishNeural stem cellTrans-differentiationTransplantationZebrafish chimera

Identifiers

PMID27554369

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.