ArticlePloS one2016
Degradation of Kidney and Psoas Muscle Proteins as Indicators of Post-Mortem Interval in a Rat Model, with Use of Lateral Flow Technology.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 2 of them syntheses that pooled it.
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Who cites it
23 citing papers in PubMed, 2 syntheses or guidelines pooled it, 39 citations in OpenAlex.
- Forensic applications of postmortem protein analysis in estimating the time since death: a systematic review.International journal of legal medicine · 2026Pooled it
- "Omics" and Postmortem Interval Estimation: A Systematic Review.International journal of molecular sciences · 2025Pooled it
- Integrated renal histopathology, RNA decay, and protein degradation signatures enhance post-mortem interval prediction using machine-learning models in a veterinary forensic rat model.Veterinary world · 2026Article
- Postmortem interval estimation based on protein analysis and marker studies in different organs in vivo.Forensic science, medicine, and pathology · 2025Article
- MMP-14 Exhibits Greater Expression, Content and Activity Compared to MMP-15 in Human Renal Carcinoma.International journal of molecular sciences · 2024Article
- The Role of Protein Degradation in Estimation Postmortem Interval and Confirmation of Cause of Death in Forensic Pathology: A Literature Review.International journal of molecular sciences · 2024Review
- Higher Content but No Specific Activity in Gelatinase B (MMP-9) Compared with Gelatinase A (MMP-2) in Human Renal Carcinoma.Cancers · 2023Article
- Trends in forensic microbiology: From classical methods to deep learning.Frontiers in microbiology · 2023Review
- A standard protocol for the analysis of postmortem muscle protein degradation: process optimization and considerations for the application in forensic PMI estimation.International journal of legal medicine · 2022Article
- Higher Content but Not Activity of Stromelysin-2 (MMP-10) in Comparison to Stromelysin-1 (MMP-3) in Human Renal Carcinoma.International journal of environmental research and public health · 2022Article
- Characterization of Exosomes and Exosomal RNAs Isolated from Post-Mortem Body Fluids for Molecular Forensic Diagnosis.Diagnostics (Basel, Switzerland) · 2022Article
- New Trends in Immunohistochemical Methods to Estimate the Time since Death: A Review.Diagnostics (Basel, Switzerland) · 2022Review
- Systematic Review on Post-Mortem Protein Alterations: Analysis of Experimental Models and Evaluation of Potential Biomarkers of Time of Death.Diagnostics (Basel, Switzerland) · 2022Review
- Novel electrochemical PMI marker biosensor based on quantum dot dissolution using a double-label strategy.Scientific reports · 2022Article
- Dismembered porcine limbs as a proxy for postmortem muscle protein degradation.International journal of legal medicine · 2021Article
- Caspase 9 and Caspase 3 Immunohistochemical Pattern in Skeletal and Cardiac Muscles at Different Times after Death: An Experimental Study on PMI Estimation.Diagnostics (Basel, Switzerland) · 2021Article
- PP2A-C may be a promising candidate for postmortem interval estimation.International journal of legal medicine · 2021Article
- Postmortem expression of apoptosis-related genes in the liver of mice and their use for estimation of the time of death.International journal of legal medicine · 2021Article
- Postmortem Protein Degradation as a Tool to Estimate the PMI: A Systematic Review.Diagnostics (Basel, Switzerland) · 2020Review
- Intra- and intermuscular variations of postmortem protein degradation for PMI estimation.International journal of legal medicine · 2020Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated potential protein markers of post-mortem interval (PMI) using rat kidney and psoas muscle. Tissue samples were taken at 12 h intervals for up to 96 h after death by suffocation. Expression levels of eight soluble proteins were analyzed by Western blotting. Degradation patterns of selected proteins were clearly divided into three groups: short-term, mid-term, and long-term PMI markers based on the half maximum intensity of intact protein expression. In kidney, glycogen synthase (GS) and glycogen synthase kinase-3β were degraded completely within 48 h making them short-term PMI markers. AMP-activated protein kinase α, caspase 3 and GS were short-term PMI markers in psoas muscle. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was a mid-term PMI marker in both tissues. Expression levels of the typical long-term PMI markers, p53 and β-catenin, were constant for at least 96 h post-mortem in both tissues. The degradation patterns of GS and caspase-3 were verified by immunohistochemistry in both tissues. GAPDH was chosen as a test PMI protein to perform a lateral flow assay (LFA). The presence of recombinant GAPDH was clearly detected in LFA and quantified in a concentration-dependent manner. These results suggest that LFA might be used to estimate PMI at a crime scene.
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