Evidence map›Paper›PMID 27552106›Full record

ArticleConnective tissue research2016

The LG/J murine strain exhibits near-normal tendon biomechanical properties following a full-length central patellar tendon defect.

Jessica R Arble, Andrea L Lalley, Nathaniel A Dyment, Pujan Joshi, Dong-Guk Shin, Cynthia Gooch, Brian Grawe, David Rowe, Jason T Shearn

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Connective tissue research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Macrophage depletion impairs neonatal tendon regeneration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Jessica R Arblea Biomedical Engineering Program, College of Engineering and Applied Science , University of Cincinnati , Cincinnati , OH , USA.
Andrea L Lalleyb Engineered Skin Laboratories , Shriners Hospital for Children , Cincinnati , OH , USA.
Nathaniel A Dymentc Department of Reconstructive Sciences, College of Dental Medicine , University of Connecticut Health Center , Farmington , CT , USA.
Pujan Joshid Computer Science and Engineering , University of Connecticut , Storrs , CT , USA.
Dong-Guk Shind Computer Science and Engineering , University of Connecticut , Storrs , CT , USA.
Cynthia Goocha Biomedical Engineering Program, College of Engineering and Applied Science , University of Cincinnati , Cincinnati , OH , USA.
Brian Grawee Department of Orthopaedic Surgery, College of Medicine , University of Cincinnati , Cincinnati , OH , USA.
David Rowec Department of Reconstructive Sciences, College of Dental Medicine , University of Connecticut Health Center , Farmington , CT , USA.
Jason T Shearna Biomedical Engineering Program, College of Engineering and Applied Science , University of Cincinnati , Cincinnati , OH , USA.
University of Cincinnati Medical Center · USUConn Health · USUniversity of Connecticut · US

Funding

A Developmentally-Based Tissue Engineering Approach to Improve Tendon RepairR01AR056943 · NIAMS · UNIVERSITY OF CINCINNATI · PI SHEARN, JASON T · 2009 to 2013
$3.3M
NIAMS NIH HHS R01 AR056943
6 · The paper itself

Abstract

purpose of the studyIdentifying biological success criteria is needed to improve therapies, and one strategy for identifying them is to analyze the RNA transcriptome for successful and unsuccessful models of tendon healing. We have characterized the MRL/MpJ murine strain and found improved mechanical outcomes following a central patellar tendon (PT) injury. In this study, we evaluate the healing of the LG/J murine strain, which comprises 75% of the MRL/MpJ background, to determine if the LG/J also exhibits improved biomechanical properties following injury and to determine differentially expressed transcription factors across the C57BL/6, MRL/MpJ and the LG/J strains during the early stages of healing. MATERIALS AND

methodsA full-length, central PT defect was created in 16-20 week old MRL/MpJ, LG/J, and C57BL/6 murine strains. Mechanical properties were assessed at 2, 5, and 8 weeks post surgery. Transcriptomic expression was assessed at 3, 7, and 14 days following injury using a novel clustering software program to evaluate differential expression of transcription factors.

resultsAverage LG/J structural properties improved to 96.7% and 97.2% of native LG/J PT stiffness and ultimate load by 8 weeks post surgery, respectively. We found the LG/J responded by increasing expression of transcription factors implicated in the inflammatory response and collagen fibril organization.

conclusionsThe LG/J strain returns to normal structural properties by 8 weeks, with steadily increasing properties at each time point. Future work will characterize the cell populations responding to injury and investigate the role of the differentially expressed transcription factors during healing.

Indexed as

AnimalsBase PairingBiomechanical PhenomenaGene Expression RegulationGene OntologyMaterials TestingMiceMice, Inbred C57BLPatellaReproducibility of ResultsSequence Analysis, RNATendonsBiomechanical propertiesmouse modeltendon healingtranscription factorstranscriptome

Identifiers

PMID27552106
PMCPMC10552235
OpenAlexW2516014606

What OpenQuestion holds

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LicenceTDM
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.