ArticleeLife2016
Patched1 and Patched2 inhibit Smoothened non-cell autonomously.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Oncogenic B-Raf proto-oncogene, serine/threonine kinase-mediated hedgehog signalling in the pathogenesis and targeted therapy of melanoma.World journal of clinical oncology · 2025Review
- Hedgehog pathway, cell cycle, and primary cilium.Cell death discovery · 2025Review
- Essential strategies for the detection of constitutive and ligand-dependent Gi-directed activity of 7TM receptors using bioluminescence resonance energy transfer.bioRxiv : the preprint server for biology · 2024Article
- Sterol regulation of developmental and oncogenic Hedgehog signaling.Biochemical pharmacology · 2022Review
- Ptch2 is a Potential Regulator of Mesenchymal Stem Cells.Frontiers in physiology · 2022Article
- Hedgehog signaling is controlled by Rac1 activity.Theranostics · 2022Article
- Generation and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Nature vs. Nurture: The Two Opposing Behaviors of Cytotoxic T Lymphocytes in the Tumor Microenvironment.International journal of molecular sciences · 2021Review
- Patched regulates lipid homeostasis by controlling cellular cholesterol levels.Nature communications · 2021Article
- Expression of Sonic Hedgehog and pathway components in the embryonic mouse head: anatomical relationships between regulators of positive and negative feedback.BMC research notes · 2021Article
- Association of Sonic Hedgehog with the extracellular matrix requires its zinc-coordination center.BMC molecular and cell biology · 2021Article
- SMO-M2 mutation does not support cell-autonomous Hedgehog activity in cerebellar granule cell precursors.Scientific reports · 2019Article
- Loss of the Heparan Sulfate Proteoglycan Glypican5 Facilitates Long-Range Sonic Hedgehog Signaling.Stem cells (Dayton, Ohio) · 2019Article
- Gain-of-function Shh mutants activate Smo cell-autonomously independent of Ptch1/2 function.Mechanisms of development · 2018Article
- Patched-2 functions to limit Patched-1 deficient skin cancer growth.Cellular oncology (Dordrecht, Netherlands) · 2018Article
- Skin-Derived Vitamin DThe Journal of investigative dermatology · 2017Article
- Emerging Roles of DYRK Kinases in Embryogenesis and Hedgehog Pathway Control.Journal of developmental biology · 2017Review
- Lipid metabolism fattens up hedgehog signaling.BMC biology · 2017Review
- New paradigms for the Hedgehog signaling network in mammary gland development and breast Cancer.Biochimica et biophysica acta. Reviews on cancer · 2017Review
- Hedgehog Signaling: From Basic Biology to Cancer Therapy.Cell chemical biology · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Smoothened (Smo) inhibition by Patched (Ptch) is central to Hedgehog (Hh) signaling. Ptch, a proton driven antiporter, is required for Smo inhibition via an unknown mechanism. Hh ligand binding to Ptch reverses this inhibition and activated Smo initiates the Hh response. To determine whether Ptch inhibits Smo strictly in the same cell or also mediates non-cell-autonomous Smo inhibition, we generated genetically mosaic neuralized embryoid bodies (nEBs) from mouse embryonic stem cells (mESCs). These experiments utilized novel mESC lines in which Ptch1, Ptch2, Smo, Shh and 7dhcr were inactivated via gene editing in multiple combinations, allowing us to measure non-cell autonomous interactions between cells with differing Ptch1/2 status. In several independent assays, the Hh response was repressed by Ptch1/2 in nearby cells. When 7dhcr was targeted, cells displayed elevated non-cell autonomous inhibition. These findings support a model in which Ptch1/2 mediate secretion of a Smo-inhibitory cholesterol precursor.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.