Evidence map›Paper›PMID 27551717›Full record

ArticlePloS one2016

A Molecular Probe for the Detection of Polar Lipids in Live Cells.

Christie A Bader, Tetyana Shandala, Elizabeth A Carter, Angela Ivask, Taryn Guinan, Shane M Hickey, Melissa V Werrett, Phillip J Wright, Peter V Simpson, Stefano Stagni and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
4.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 37 citations in OpenAlex.

  1. Review
  2. Article
  3. Analytical Techniques for Single-Cell Biochemical Assays of Lipids.Annual review of biomedical engineering · 2023
    Review
  4. Rhenium(I) conjugates as tools for tracking cholesterol in cells.Metallomics : integrated biometal science · 2022
    Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 2 countries.

Christie A BaderSchool of Pharmacy and Medical Science, University of South Australia, Adelaide, South Australia, Australia.
Tetyana ShandalaSchool of Pharmacy and Medical Science, University of South Australia, Adelaide, South Australia, Australia.
Elizabeth A CarterVibrational Spectroscopy Core Facility, The University of Sydney, Sydney, New South Wales, Australia.
Angela IvaskFuture Industries Institute, University of South Australia, Mawson Lakes, South Australia, Australia.
Taryn GuinanFuture Industries Institute, University of South Australia, Mawson Lakes, South Australia, Australia.
Shane M HickeySchool of Pharmacy and Medical Science, University of South Australia, Adelaide, South Australia, Australia.
Melissa V WerrettDepartment of Chemistry and Nanochemistry Research Institute, Curtin University, Bentley, Western Australia, Australia.
Phillip J WrightDepartment of Chemistry and Nanochemistry Research Institute, Curtin University, Bentley, Western Australia, Australia.
Peter V SimpsonDepartment of Chemistry and Nanochemistry Research Institute, Curtin University, Bentley, Western Australia, Australia.
Stefano StagniDepartment of Industrial Chemistry "Toso Montanari", University of Bologna, Bologna, Italy.
Nicolas H VoelckerFuture Industries Institute, University of South Australia, Mawson Lakes, South Australia, Australia.
Peter A LayVibrational Spectroscopy Core Facility, The University of Sydney, Sydney, New South Wales, Australia.
Massimiliano MassiDepartment of Chemistry and Nanochemistry Research Institute, Curtin University, Bentley, Western Australia, Australia.
Sally E PlushSchool of Pharmacy and Medical Science, University of South Australia, Adelaide, South Australia, Australia.ORCID http://orcid.org/0000-0002-9999-9154
Douglas A BrooksSchool of Pharmacy and Medical Science, University of South Australia, Adelaide, South Australia, Australia.
University of South Australia · AUCurtin University · AUThe University of Sydney · AUUniversity of Bologna · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lipids have an important role in many aspects of cell biology, including membrane architecture/compartment formation, intracellular traffic, signalling, hormone regulation, inflammation, energy storage and metabolism. Lipid biology is therefore integrally involved in major human diseases, including metabolic disorders, neurodegenerative diseases, obesity, heart disease, immune disorders and cancers, which commonly display altered lipid transport and metabolism. However, the investigation of these important cellular processes has been limited by the availability of specific tools to visualise lipids in live cells. Here we describe the potential for ReZolve-L1™ to localise to intracellular compartments containing polar lipids, such as for example sphingomyelin and phosphatidylethanolamine. In live Drosophila fat body tissue from third instar larvae, ReZolve-L1™ interacted mainly with lipid droplets, including the core region of these organelles. The presence of polar lipids in the core of these lipid droplets was confirmed by Raman mapping and while this was consistent with the distribution of ReZolve-L1™ it did not exclude that the molecular probe might be detecting other lipid species. In response to complete starvation conditions, ReZolve-L1™ was detected mainly in Atg8-GFP autophagic compartments, and showed reduced staining in the lipid droplets of fat body cells. The induction of autophagy by Tor inhibition also increased ReZolve-L1™ detection in autophagic compartments, whereas Atg9 knock down impaired autophagosome formation and altered the distribution of ReZolve-L1™. Finally, during Drosophila metamorphosis fat body tissues showed increased ReZolve-L1™ staining in autophagic compartments at two hours post puparium formation, when compared to earlier developmental time points. We concluded that ReZolve-L1™ is a new live cell imaging tool, which can be used as an imaging reagent for the detection of polar lipids in different intracellular compartments.

Indexed as

Cell Physiological PhenomenaLipid MetabolismLipidsMolecular ProbesAdipocytesAdipose TissueAmino AcidsAnimalsAutophagyBiological TransportCarbohydrate MetabolismDrosophilaLipid DropletsMetamorphosis, BiologicalMiceSpectrum Analysis, RamanAmino AcidsLipidsMolecular Probes

Identifiers

PMID27551717
PMCPMC4994960
OpenAlexW2512267046

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.