Evidence map›Paper›PMID 27551542›Full record

ArticleCell death discovery2016

Retinal pigment epithelial cell necroptosis in response to sodium iodate.

J Hanus, C Anderson, D Sarraf, J Ma, S Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell death discovery, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.

0numbers the graph read from it
0cells of the map it votes in
109citing papers in PubMed
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

109 citing papers in PubMed, 157 citations in OpenAlex.

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49 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

J HanusDepartment of Cell and Molecular Biology, Tulane University , 2000 Percival Stern Hall, 6400 Freret Street, New Orleans, LA 70118, USA.
C AndersonDepartment of Cell and Molecular Biology, Tulane University , 2000 Percival Stern Hall, 6400 Freret Street, New Orleans, LA 70118, USA.
D SarrafDepartment of Cell and Molecular Biology, Tulane University , 2000 Percival Stern Hall, 6400 Freret Street, New Orleans, LA 70118, USA.
J MaDepartment of Cell and Molecular Biology, Tulane University , 2000 Percival Stern Hall, 6400 Freret Street, New Orleans, LA 70118, USA.
S WangDepartment of Cell and Molecular Biology, Tulane University, 2000 Percival Stern Hall, 6400 Freret Street, New Orleans, LA 70118, USA; Department of Ophthalmology, Tulane University, 1430 Tulane Avenue, SL-69, New Orleans, LA 70112, USA.
Tulane University · US

Funding

Regulation of Ocular Angiogenesis by microRNAsR01EY021862 · NEI · UT SOUTHWESTERN MEDICAL CENTER · PI WANG, SHUSHENG · 2011 to 2021
$2.8M
NEI NIH HHS R01 EY021862
6 · The paper itself

Abstract

Age-related macular degeneration (AMD) is a degenerative disease of the retina and the leading cause of blindness in the elderly in developed countries. The late stage of dry AMD, or geographic atrophy (GA), is characterized by extensive retinal pigment epithelium (RPE) degeneration. The underlying molecular mechanism for RPE cell death in GA remains unclear. Our previous study has established that RPE cells die predominantly from necroptosis in response to oxidative stress in vitro. Here, we extend our study and aim to characterize the nature of RPE cell death in response to sodium iodate (NaIO3) in vitro and in a NaIO3-induced retina degeneration mouse model. We found that NaIO3 induces RPE necroptosis in vitro by using a combination of molecular hallmarks. By using TUNEL assays, active caspase-3 and HMGB1 immunostaining, we confirmed that photoreceptor cells die mainly from apoptosis and RPE cells die mainly from necroptosis in response to NaIO3 in vivo. RPE necroptosis in this model is also supported by use of the RIPK1 inhibitor, Necrostatin-1. Furthermore, using novel RIPK3-GFP transgenic mouse lines, we detected RIPK3 aggregation, a hallmark of necroptosis, in the RPE cells in vivo after NaIO3 injection. Our findings suggest the necessity of re-evaluating RPE cell death mechanism in AMD models and have the potential to influence therapeutic development for dry AMD, especially GA.

Identifiers

PMID27551542
PMCPMC4979458
OpenAlexW2472557182

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.