ArticleThe Journal of cell biology2016
RhoA GTPase inhibition organizes contraction during epithelial morphogenesis.
Article in The Journal of cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed.
- Antioxidant enhancement and myopia progression delay by Rho-kinase inhibition in guinea pig retina.Molecular biology reports · 2026Article
- Systematic analysis of RhoGAP expression and function in border cell morphology and migration.bioRxiv : the preprint server for biology · 2026Article
- MIM triggers formin to Arp2/3-based actin assembly in membrane remodeling in Drosophila embryos.The Journal of cell biology · 2026Article
- An active matter model captures spatial dynamics of actomyosin oscillations in larval epithelial cells duringProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Reprogramming of Human Limbal Niche Progenitors.International journal of medical sciences · 2026Review
- Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue furrowing.Nature communications · 2025Article
- Apical constriction requires patterned apical surface remodeling to synchronize cellular deformation.eLife · 2025Article
- Anillin tunes contractility and regulates barrier function during Rho flare-mediated tight junction remodeling.Molecular biology of the cell · 2025Article
- Anillin tunes contractility and regulates barrier function during Rho flare-mediated tight junction remodeling.bioRxiv : the preprint server for biology · 2024Article
- Regulation of intercellular viscosity by E-cadherin-dependent phosphorylation of EGFR in collective cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Change in RhoGAP and RhoGEF availability drives transitions in cortical patterning and excitability in Drosophila.Current biology : CB · 2024Article
- Article
- Rhoa/ROCK, mTOR and Secretome-Based Treatments for Ischemic Stroke: New Perspectives.Current issues in molecular biology · 2024Review
- Patterning of the cell cortex by Rho GTPases.Nature reviews. Molecular cell biology · 2024Review
- Medioapical contractile pulses coordinated between cells regulate Drosophila eye morphogenesis.The Journal of cell biology · 2024Article
- Reshaping the Syncytial Drosophila Embryo with Cortical Actin Networks: Four Main Steps of Early Development.Results and problems in cell differentiation · 2024Review
- The dynamics and biophysics of shape formation: Common themes in plant and animal morphogenesis.Developmental cell · 2023Review
- Change in RhoGAP and RhoGEF availability drives transitions in cortical patterning and excitability inbioRxiv : the preprint server for biology · 2023Article
- Actomyosin pulsing rescues embryonic tissue folding from disruption by myosin fluctuations.Research square · 2023Article
- Early zygotic gene product Dunk interacts with anillin to regulate Myosin II duringMolecular biology of the cell · 2023Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
During morphogenesis, contraction of the actomyosin cytoskeleton within individual cells drives cell shape changes that fold tissues. Coordination of cytoskeletal contractility is mediated by regulating RhoA GTPase activity. Guanine nucleotide exchange factors (GEFs) activate and GTPase-activating proteins (GAPs) inhibit RhoA activity. Most studies of tissue folding, including apical constriction, have focused on how RhoA is activated by GEFs to promote cell contractility, with little investigation as to how GAPs may be important. Here, we identify a critical role for a RhoA GAP, Cumberland GAP (C-GAP), which coordinates with a RhoA GEF, RhoGEF2, to organize spatiotemporal contractility during Drosophila melanogaster apical constriction. C-GAP spatially restricts RhoA pathway activity to a central position in the apical cortex. RhoGEF2 pulses precede myosin, and C-GAP is required for pulsation, suggesting that contractile pulses result from RhoA activity cycling. Finally, C-GAP expression level influences the transition from reversible to irreversible cell shape change, which defines the onset of tissue shape change. Our data demonstrate that RhoA activity cycling and modulating the ratio of RhoGEF2 to C-GAP are required for tissue folding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.